Oliveira S A, Fontanelli B A F, Stefanini M A, Chuffa L G A, Teixeira G R, Lizarte F S N, Tirapelli L F, Quitete V H A, Matheus S M M, Padovani C R, Martinez M, Martinez F E
Department of Anatomy, Biosciences Institute, UNESP-Univ. Estadual Paulista, Botucatu, SP, Brazil.
Microsc Res Tech. 2014 Jan;77(1):44-51. doi: 10.1002/jemt.22311. Epub 2013 Nov 6.
Maternal care is the main source of signals and stimuli for proper development, growth, and production of adjustment responses to stressful factors. Adverse experiences in childhood are associated with a vulnerability to developing abusive ethanol ingestion via alterations of the response of the hypothalamic-pituitary-adrenal axis. Alcoholism causes global brain abnormalities, with the cerebellum being one of the most susceptible areas. We evaluated the effect of maternal separation on the cerebellum structure of male UCh rats. Adult male UChA (low 10% ethanol consumption) and UChB (high 10% ethanol consumption) rats were divided in to four experimental groups: (1) UChA, (2) UChA maternal separation (MS), (3) UChB, and (4) UChB MS. The MS occurred between the 4th and 14th days of age, for 240 min day(-1) . Euthanasia was performed at 120 days of age. An image analysis system was used to measure cerebellar cortical height and Purkinje cellular area and height in five rats from each group. The cerebellar sections were stained with antibodies against IGFR-I. MS did not alter the ethanol consumption of UChA and UChB rats. Corticosterone level was significantly higher in UChA MS and UChB MS rats than in UChA and UChB rats. The Purkinje cellular area and height were higher in UChA MS rats. IGFR-I expression was observed in the cortical glomerular area of UChA MS and UChB MS rats. MS altered the Purkinje cells in the cerebella of male UCh rats.
母性关怀是正常发育、生长以及产生对应激因素的调节反应的主要信号和刺激来源。童年时期的不良经历与下丘脑 - 垂体 - 肾上腺轴反应改变导致滥用乙醇摄入的易感性相关。酒精中毒会导致全脑异常，小脑是最易受影响的区域之一。我们评估了母鼠分离对雄性UCh大鼠小脑结构的影响。成年雄性UChA（低10%乙醇摄入量）和UChB（高10%乙醇摄入量）大鼠被分为四个实验组：（1）UChA，（2）UChA母鼠分离（MS），（3）UChB，和（4）UChB MS。母鼠分离发生在出生后第4天至第14天，每天240分钟。在120日龄时实施安乐死。使用图像分析系统测量每组五只大鼠的小脑皮质高度、浦肯野细胞面积和高度。小脑切片用抗IGFR - I抗体染色。母鼠分离并未改变UChA和UChB大鼠的乙醇摄入量。UChA MS和UChB MS大鼠的皮质酮水平显著高于UChA和UChB大鼠。UChA MS大鼠的浦肯野细胞面积和高度更高。在UChA MS和UChB MS大鼠的皮质肾小球区域观察到IGFR - I表达。母鼠分离改变了雄性UCh大鼠小脑的浦肯野细胞。
