Chuffa Luiz Gustavo A, Padovani Carlos R, Martinez Francisco E
Department of Cell Biology, Institute of Biology, State University of Campinas-UNICAMP, Campinas-SP, Brazil.
Maturitas. 2009 Jan 20;62(1):21-9. doi: 10.1016/j.maturitas.2008.09.027. Epub 2008 Nov 18.
In females, chronic alcoholism has a current and dangerous incidence to fertility. This work had the goal of elucidating the alterations on the ovary of UChA and UChB adult rats (ethanol 10% (v/v) voluntary drinkers).
After the treatment period, 42 female rats divided into three experimental groups (UChA, UChB and Wistar) suffered decapitation and their ovaries were removed and processed to further analysis on light and electron microscopy. The ovary was entirely sliced and stained by hematoxylin-eosin, toluidine blue, periodic acid Schiff (PAS) and Masson's tricromic. Thereby, the enzymatic reaction to acid and alkaline phosphatase, estral cyclicity, reproductive hormonal status and frequency in oestrous-related ovarian structures were assigned.
The UChB rats showed an increase in body mass gain index and the ovaries relative weight was significantly lower comparing to the other groups. UCh rats presented the longest estral cycle durations and also persistent oestrous phasis, with uninterrupted cycles. Advanced follicular atresia was common in UCh animals, and degenerating intracellular fragments could be observed through acid phosphatase and electron microscopy techniques.
There were some estral cyclicity irregularities caused by chronic ethanol intake in the UCh groups which were consequently reflected as morphologic injury in the ovary structure.
在女性中，慢性酒精中毒对生育能力有当前且危险的影响。本研究旨在阐明成年UChA和UChB大鼠（自愿饮用10%（v/v）乙醇）卵巢的变化。
在治疗期结束后，将42只雌性大鼠分为三个实验组（UChA、UChB和Wistar），进行断头处理，取出卵巢并进行处理，以便进行光镜和电镜进一步分析。将卵巢完全切片，用苏木精-伊红、甲苯胺蓝、过碘酸希夫（PAS）和马森三色染色。从而确定对酸性和碱性磷酸酶的酶促反应、发情周期、生殖激素状态以及与发情相关的卵巢结构的频率。
UChB大鼠体重增加指数升高，与其他组相比，卵巢相对重量显著降低。UCh大鼠的发情周期持续时间最长，且处于持续发情阶段，周期不间断。UCh组动物中晚期卵泡闭锁常见，通过酸性磷酸酶和电镜技术可观察到细胞内碎片退化。
UCh组中慢性乙醇摄入导致了一些发情周期不规则，进而反映为卵巢结构的形态学损伤。
