Núcleo de Bioensaios, Biossíntese e Ecofisiologia de Produtos Naturais, Departamento de Química Orgânica, Instituto de Química, Universidade Estadual Paulista, 14801-970 Araraquara, SP, Brazil.
Biomed Res Int. 2013;2013:485837. doi: 10.1155/2013/485837. Epub 2013 Sep 25.
Establishment of adventitious root cultures of Peritassa campestris (Celastraceae) was achieved from seed cotyledons cultured in semisolid Woody Plant Medium (WPM) supplemented with 2% sucrose, 0.01% PVP, and 4.0 mg L⁻¹ IBA. Culture period on accumulation of biomass and quinone-methide triterpene maytenin in adventitious root were investigated. The accumulation of maytenin in these roots was compared with its accumulation in the roots of seedlings grown in a greenhouse (one year old). A rapid detection and identification of maytenin by direct injection into an atmospheric-pressure chemical ionization ion trap tandem mass spectrometer (APCI-IT-MS/MS) were performed without prior chromatographic separation. In vitro, the greatest accumulation of biomass occurred within 60 days of culture. The highest level of maytenin--972.11 μ g·g⁻¹ dry weight--was detected at seven days of cultivation; this value was 5.55-fold higher than that found in the roots of seedlings grown in a greenhouse.
建立了 Peritassa campestris（卫矛科）不定根培养物，方法是将种子子叶培养在半固体 Woody Plant Medium（WPM）中，添加 2%蔗糖、0.01%PVP 和 4.0mg·L⁻¹ IBA。研究了不定根生物量和醌甲烯三萜马滕宁积累的培养期。将这些根中的马滕宁的积累与在温室中生长的幼苗（一岁）的根中的积累进行了比较。通过直接注入大气压化学电离离子阱串联质谱仪（APCI-IT-MS/MS）进行了马滕宁的快速检测和鉴定，而无需预先进行色谱分离。在体外，培养 60 天内生物量积累最大。培养七天时检测到最高水平的马滕宁-972.11μ g·g⁻¹干重-这一数值比在温室中生长的幼苗的根中的含量高 5.55 倍。
