Pina Edieidia S, Silva Denise B, Teixeira Simone P, Coppede Juliana S, Furlan Maysa, França Suzelei C, Lopes Norberto P, Pereira Ana Maria S, Lopes Adriana A
Unidade de Biotecnologia, Universidade de Ribeirão Preto, Av. Costábile Romano, 2201, 14096-900, Ribeirão Preto, SP, Brazil.
Núcleo de Pesquisa em Produtos Naturais e Sintéticos (NPPNS), Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14040-903, Brazil.
Sci Rep. 2016 Mar 4;6:22627. doi: 10.1038/srep22627.
Biosynthetic investigation of quinonemethide triterpenoid 22β-hydroxy-maytenin (2) from in vitro root cultures of Peritassa laevigata (Celastraceae) was conducted using (13)C-precursor. The mevalonate pathway in P. laevigata is responsible for the synthesis of the quinonemethide triterpenoid scaffold. Moreover, anatomical analysis of P. laevigata roots cultured in vitro and in situ showed the presence of 22β-hydroxy-maytenin (2) and maytenin (1) in the tissues from transverse or longitudinal sections with an intense orange color. MALDI-MS imaging confirmed the distribution of (2) and (1) in the more distal portions of the root cap, the outer cell layers, and near the vascular cylinder of P. laevigata in vitro roots suggesting a role in plant defense against infection by microorganisms as well as in the root exudation processes.
利用¹³C前体对光叶围盘树（卫矛科）离体根培养物中的醌甲基三萜类化合物22β-羟基美登木素（2）进行了生物合成研究。光叶围盘树中的甲羟戊酸途径负责醌甲基三萜类骨架的合成。此外，对光叶围盘树离体和原位培养根的解剖分析表明，在横切或纵切组织中存在22β-羟基美登木素（2）和美登木素（1），颜色呈强烈的橙色。基质辅助激光解吸电离质谱成像证实了（2）和（1）在光叶围盘树离体根的根冠更远端部分、外细胞层以及维管束柱附近的分布，表明其在植物抵御微生物感染以及根分泌过程中发挥作用。
