Mikoshiba K, Takamatsu K, Tsukada Y
Dev Neurosci. 1985;7(4):199-205. doi: 10.1159/000112288.
The cerebral cortex of reeler mutant mice characterized by the derangement of cell position was analyzed by an immunohistochemical staining technique using antiserum against myelin basic protein. All the myelins of the myelinated fibers were stained, so that the fiber trajectories could be clearly traced. In the present study the changes in the fiber trajectories were accompanied by changes in myelination sites. Myelinated fibers were localized in the central white matter and gradually became sparser toward the surface of the normal cerebral cortex, while in the reeler myelinated fibers were observed throughout the cortical plane. From the immunohistochemical analysis of the changes occurring in the myelinated fiber pathways in developmental sequence, it was also shown that the fiber bundle accumulation was thin at the earlier stages (e.g. the 15th postnatal day) and became thick as the animal grew, and the timing of myelination in the reeler was similar to that of the control.
利用抗髓磷脂碱性蛋白抗血清，通过免疫组织化学染色技术对以细胞位置紊乱为特征的reeler突变小鼠的大脑皮质进行了分析。所有有髓纤维的髓磷脂都被染色，从而可以清晰地追踪纤维轨迹。在本研究中，纤维轨迹的变化伴随着髓鞘形成部位的变化。有髓纤维位于正常大脑皮质中央白质，并朝着皮质表面逐渐变得稀疏，而在reeler小鼠中，有髓纤维在整个皮质平面均有观察到。从对发育过程中有髓纤维通路发生的变化进行的免疫组织化学分析还可以看出，在早期阶段（如出生后第15天）纤维束堆积较薄，随着动物生长而变厚，且reeler小鼠的髓鞘形成时间与对照组相似。
