Mikoshiba K, Terada S, Takamatsu K, Shimai K, Tsukada Y
Dev Neurosci. 1983;6(2):101-10. doi: 10.1159/000112336.
The organization of the fiber connections and architecture of the cerebellum of reeler mutant mice was analyzed by an immunohistochemical and histochemical procedure. By immunohistochemical staining of the myelinated fiber arborization with antiserum against myelin basic protein, it was found in the reeler cerebellum that the fibers ran in all directions throughout the white matter. Some of the fibers surrounded Purkinje cells. The distribution of AChE-positive fibers was abnormal and some Purkinje cells surrounded by AChE-positive fibers were present. Molecular layers and glomeruli showed strong succinic dehydrogenase (SDH) activity. The white matter of the reeler cerebellum showed a mosaic of SDH-positive and -negative sites. The results indicate that changes in the distribution of myelinated fiber arborization and the change in the distribution of SDH and AChE activity are different in each part of the cerebellum as a result of the disorganization of the architecture of the reeler mutation.
通过免疫组织化学和组织化学方法分析了reeler突变小鼠小脑的纤维连接组织和结构。用抗髓磷脂碱性蛋白抗血清对有髓纤维分支进行免疫组织化学染色，发现在reeler小鼠小脑中，纤维在整个白质中向各个方向延伸。一些纤维围绕着浦肯野细胞。乙酰胆碱酯酶（AChE）阳性纤维的分布异常，存在一些被AChE阳性纤维围绕的浦肯野细胞。分子层和小球显示出强烈的琥珀酸脱氢酶（SDH）活性。reeler小鼠小脑的白质呈现出SDH阳性和阴性区域的镶嵌。结果表明，由于reeler突变导致结构紊乱，有髓纤维分支分布的变化以及SDH和AChE活性分布的变化在小脑的各个部分有所不同。
