Tanaka Noriko, Manabe Masahiro, Yamashita Shouichirou, Ikeda Katsuyoshi, Obayashi Konen, Uchiba Mituhiro, Ando Yukio
Department of Laboratory Medicine, Kumamoto University Hospital, Kumamoto 860-8556, Japan.
Rinsho Byori. 2013 Jul;61(7):576-82.
APTT is widely used for serial monitoring of treatment with unfractionated heparin. However, since its sensitivity to heparin varies significantly from one reagent to another, it is suggested that the therapeutic range had to be defined for each brand of APTT reagents. In this study, to investigate the affect of these variables, we compared various reagents of APTT and instruments for APTT measurements in different laboratories of university hospitals and related hospitals in Kyushu area. Same sample of normal pooled plasma added unfractionated heparin were measured in each laboratory. Prolongation of APTT by unfractionated heparin differed between laboratories. In addition, we examined prolongation of APTT by unfractionated heparin in normal plasma obtained from single donor. The prolongation of APTT differed between single donor samples even in the using of single APTT reagent and instrument. No correlation was observed between prolongation ratio of APTT and antithrombin concentration. These results indicate that sensitivity to heparin varies between individual in addition to brand of APTT reagent.
活化部分凝血活酶时间（APTT）广泛用于普通肝素治疗的系列监测。然而，由于其对肝素的敏感性在不同试剂之间差异显著，建议针对每个品牌的APTT试剂确定治疗范围。在本研究中，为了调查这些变量的影响，我们在九州地区大学医院和相关医院的不同实验室中比较了各种APTT试剂和用于APTT测量的仪器。在每个实验室中对添加了普通肝素的相同正常混合血浆样本进行测量。普通肝素导致的APTT延长在不同实验室之间存在差异。此外，我们检测了从单一供体获得的正常血浆中普通肝素导致的APTT延长情况。即使使用单一的APTT试剂和仪器，单一供体样本之间的APTT延长也存在差异。未观察到APTT延长率与抗凝血酶浓度之间的相关性。这些结果表明，除了APTT试剂品牌外，个体之间对肝素的敏感性也存在差异。
