Dunkley M L, Husband A J
Immunology. 1986 Mar;57(3):379-85.
The distribution of keyhole limpet haemocyanin (KLH)-specific helper cells for antibody responses of IgA, IgM and IgG isotypes in Peyer's patch (PP), mesenteric lymph node (MLN) and peripheral lymph node (PLN) was examined following oral, intraduodenal (ID), intraperitoneal (IP), intra-Peyer's patch (IPP) or subcutaneous (SC) immunization with KLH. Oral or ID immunization gave little or no response in any tissue studied. IP immunization with or without a subsequent ID challenge gave rise to a modest IgA and IgM helper response in MLN but a small IgA and IgM helper response in PP and PLN. IP immunization alone did not stimulate IgG-specific help in any tissues studied, but a small IgG helper response occurred in MLN and PLN after subsequent ID challenge. IPP was the most effective route of immunization, giving rise to a large helper response for IgA, IgM and IgG isotypes in PP, a smaller response in MLN and no response in PLN. The helper response following IPP immunization was not augmented by subsequent ID challenge. SC immunization gave a small but significant helper response for all isotypes in PLN but no response in PP or MLN. The kinetics of the helper response were examined in PP, MLN, PLN and thoracic duct lymph (TDL) following IPP immunization. The helper response for all isotypes in PP was maximal at 2 weeks and then declined. Similar kinetics but of lower magnitude were observed in MLN and TDL. The presence of IgA-specific helper cells in TDL demonstrates that these cells migrate, presumably from GALT, and may constitute an important component of mucosal responses at extraintestinal sites.
在用钥孔戚血蓝蛋白（KLH）进行口服、十二指肠内（ID）、腹腔内（IP）、派伊尔结内（IPP）或皮下（SC）免疫后，检测了派伊尔结（PP）、肠系膜淋巴结（MLN）和外周淋巴结（PLN）中针对IgA、IgM和IgG同种型抗体反应的KLH特异性辅助细胞的分布情况。口服或ID免疫在任何研究的组织中几乎没有或没有反应。IP免疫无论是否随后进行ID激发，在MLN中均引起适度的IgA和IgM辅助反应，但在PP和PLN中引起较小的IgA和IgM辅助反应。单独的IP免疫在任何研究的组织中均未刺激IgG特异性辅助反应，但在随后的ID激发后，MLN和PLN中出现了较小的IgG辅助反应。IPP是最有效的免疫途径，在PP中对IgA、IgM和IgG同种型产生大量辅助反应，在MLN中反应较小，在PLN中无反应。IPP免疫后的辅助反应不会因随后的ID激发而增强。SC免疫在PLN中对所有同种型产生小但显著的辅助反应，但在PP或MLN中无反应。在IPP免疫后，检测了PP、MLN、PLN和胸导管淋巴（TDL）中辅助反应的动力学。PP中所有同种型的辅助反应在2周时达到最大值，然后下降。在MLN和TDL中观察到类似的动力学，但幅度较低。TDL中存在IgA特异性辅助细胞表明这些细胞可能从肠道相关淋巴组织迁移而来，并可能构成肠外部位黏膜反应的重要组成部分。
