Endoh M, Sakai H, Nomoto Y, Tomino Y, Kaneshige H
J Immunol. 1981 Dec;127(6):2612-3.
A subpopulation of human T lymphocytes that express receptors of immunoglobulin A (T alpha cells) was separated by a FACS using FITC-conjugated human IgA myeloma protein in order to determine whether they have helper activity for in vitro immunoglobulin synthesis in comparison with a T alpha cell population separated by rosette formation using PTN-ORBC conjugated with MOPC-315 mouse IgA myeloma protein. FACS-separated T alpha cells have IgA specific helper activity, whereas rosette-separated T alpha cells have polyclonal helper activity for in vitro immunoglobulin synthesis in PWM-stimulated B cells. The polyclonal helper activity observed in rosette-separated T alpha cells might be due to contamination of non-T alpha cells in the T alpha cell fraction. It is concluded that purified T alpha cells in normal human peripheral blood show IgA-specific helper activity in vitro.
利用异硫氰酸荧光素（FITC）偶联的人免疫球蛋白A骨髓瘤蛋白，通过荧光激活细胞分选仪（FACS）分离出表达免疫球蛋白A受体的人T淋巴细胞亚群（Tα细胞），目的是与使用与MOPC - 315小鼠免疫球蛋白A骨髓瘤蛋白偶联的PTN - ORBC通过玫瑰花结形成分离的Tα细胞群体相比，确定它们对体外免疫球蛋白合成是否具有辅助活性。FACS分离的Tα细胞具有IgA特异性辅助活性，而玫瑰花结分离的Tα细胞对PWM刺激的B细胞体外免疫球蛋白合成具有多克隆辅助活性。在玫瑰花结分离的Tα细胞中观察到的多克隆辅助活性可能是由于Tα细胞组分中存在非Tα细胞污染。结论是正常人外周血中纯化的Tα细胞在体外表现出IgA特异性辅助活性。
