Smith R J, Bowman B J, Speziale S C
Int J Immunopharmacol. 1986;8(1):33-40. doi: 10.1016/0192-0561(86)90070-6.
The interaction of human polymorphonuclear neutrophilic leukocytes (neutrophils) with interleukin-1 (IL-1) resulted in a time- and concentration-dependent, selective, release of azurophil (myeloperoxidase, lysozyme) and specific (lysozyme, vitamin B12-binding protein) granule constituents. Myeloperoxidase (MPO) and lysozyme secretion was markedly attenuated if neutrophils were not exposed to cytochalasin B (CB) prior to contact with IL-1. Degranulation was significantly enhanced in the presence of extracellular calcium. IL-1-elicited granule exocytosis was inhibited by the intracellular calcium antagonist, 8-(N,N-diethylamino)-octyl-(3,4,5-trimethoxy) benzoate hydrochloride (TMB-8), a calmodulin antagonist, trifluoperazine (TFP), and an anion channel blocker, 4,4'-diisothiocyano-2,2'-disulfonic acid stilbene (DIDS). An evaluation of the role of arachidonic acid metabolites in IL-1-induced neutrophil activation revealed a suppressive effect on enzyme release exerted by the lipoxygenase inhibitors, piriprost potassium (6,9,deepoxy-6,9-(phenylimino)-delta 6,8 -prostaglandin I1, U-60,257B) and NDGA (nordihydroguaiaretic acid), and a cyclooxygenase/lipoxygenase inhibitor, ETYA (5,8,11,14-eicosatetraynoic acid). These data describe the characteristics of IL-1 as a human neutrophil secretagogue, and enhance our insight into the mechanism of inflammatory cell activation with this monokine.
人类多形核嗜中性白细胞(中性粒细胞)与白细胞介素-1(IL-1)相互作用,导致嗜天青颗粒(髓过氧化物酶、溶菌酶)和特异性颗粒(溶菌酶、维生素B12结合蛋白)成分呈时间和浓度依赖性、选择性释放。如果中性粒细胞在与IL-1接触之前未暴露于细胞松弛素B(CB),则髓过氧化物酶(MPO)和溶菌酶的分泌会显著减弱。在细胞外钙存在的情况下,脱颗粒显著增强。细胞内钙拮抗剂盐酸8-(N,N-二乙氨基)-辛基-(3,4,5-三甲氧基)苯甲酸酯(TMB-8)、钙调蛋白拮抗剂三氟拉嗪(TFP)和阴离子通道阻滞剂4,4'-二异硫氰酸-2,2'-二磺酸芪(DIDS)可抑制IL-1诱导的颗粒胞吐作用。对花生四烯酸代谢产物在IL-1诱导的中性粒细胞活化中的作用进行评估发现,脂氧合酶抑制剂吡咯前列素钾(6,9,去氧-6,9-(苯基亚氨基)-δ6,8-前列腺素I1,U-60,257B)和去甲二氢愈创木酸(NDGA)以及环氧化酶/脂氧合酶抑制剂5,8,11,14-二十碳四炔酸(ETYA)对酶释放具有抑制作用。这些数据描述了IL-1作为人类中性粒细胞促分泌素的特性,并增强了我们对这种单核因子介导的炎症细胞活化机制的理解。