Smith R J, Bowman B J, Iden S S
Biochem Biophys Res Commun. 1984 May 16;120(3):964-72. doi: 10.1016/s0006-291x(84)80201-6.
We investigated the capacity of DIDS to influence degranulation and superoxide anion (O-2) generation by human neutrophils exposed to soluble, surface-active stimuli. DIDS caused a concentration-related (25-200 microM) inhibition of myeloperoxidase (MPO) release from N-formyl-methionyl-leucyl-phenylalanine (FMLP), 5(S), 12(R)-dihydroxy-6, 14-cis-8, 10-trans-eicosatetraenoic acid (LTB4), 1-0-hexadecyl/octadecyl-2-0-acetyl-sn-glyceryl-3-phosphorylcholine (AGEPC) and phorbol myristate acetate (PMA)-stimulated neutrophils. In contrast, DIDS had no effect on O-2 production by FMLP and PMA activated cells, whereas it enhanced LTB4 and AGEPC-elicited O-2 generation. The respective effects of DIDS on these neutrophil activities were reversed by washing the cells prior to exposure to stimulus. Thus, DIDS represents a useful pharmacologic probe for investigating the role(s) of anions in the mechanisms of neutrophil activation with structurally and chemically dissimilar stimuli.
我们研究了二异丙基氨基磺酸钠（DIDS）对暴露于可溶性表面活性刺激物的人中性粒细胞脱颗粒和超氧阴离子（O₂⁻）生成的影响。DIDS对N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）、5(S),12(R)-二羟基-6,14-顺-8,10-反-二十碳四烯酸（LTB4）、1-油酰基/硬脂酰基-2-乙酰基-sn-甘油-3-磷酸胆碱（AGEPC）和佛波酯（PMA）刺激的中性粒细胞中髓过氧化物酶（MPO）的释放产生浓度相关（25-200微摩尔）的抑制作用。相比之下，DIDS对FMLP和PMA激活的细胞产生O₂⁻没有影响，而它增强了LTB4和AGEPC引发的O₂⁻生成。在暴露于刺激物之前洗涤细胞可逆转DIDS对这些中性粒细胞活性的各自影响。因此，DIDS是一种有用的药理学探针，用于研究阴离子在结构和化学性质不同的刺激物激活中性粒细胞机制中的作用。
