Coeshott C M, Chesnut R W, Kubo R T, Grammer S F, Jenis D M, Grey H M
J Immunol. 1986 Apr 15;136(8):2832-8.
This study was undertaken to determine the nature of the antigens recognized in allogeneic and syngeneic mixed leukocyte reactions (MLR). Specifically, we wished to determine whether Ia antigens alone were recognized by MLR-reactive T cells, or whether the specificity was determined by the corecognition of non-MHC antigens together with syngeneic or allogeneic Ia. To do this we used 11 T cell hybrids that were characterized as being specific for Iad and were tested their capacity to respond to isolated I-Ad or I-Ed that had been incorporated into liposomes and had bound to the surface of glass beads. Of nine alloreactive T cell hybrids (five I-Ad-and four I-Ed-specific), seven were shown to be responsive to the relevant isolated Ia antigen on glass beads. Also, two of two syngeneic I-Ad-specific T cell hybrids responded to I-Ad on the glass beads. One of the two alloreactive T cell hybrids that failed to respond to the relevant Ia antigen on glass beads was shown to be specific for an antigen in fetal calf serum (FCS) that was recognized in the context of the allo-Ia antigen (I-Ed), because when intact accessory cells were used, a response by this hybrid was only observed when FCS was present in the assay culture medium or when the accessory cells were pre-pulsed with FCS. The possible involvement of FCS antigens and non-Ia accessory cell antigens in the stimulation of the nine T cell hybrids that responded to isolated Ia on glass beads was evaluated. T cell hybrids that were grown and were tested in serum free medium were still capable of reacting to Ia on beads. The isolated Ia preparations used were greater than 90% pure, and their capacity to stimulate the T cell hybrids did not correlate with the degree of contamination with non-Ia proteins. We conclude from these studies that the majority of T cells that respond to allogeneic or syngeneic Ia bearing stimulator cells are specific for the Ia antigens themselves, and do not require the co-recognition of other non-Ia antigens; nor is there any requirement for Ia antigen processing for this recognition.
本研究旨在确定在同种异体和同基因混合淋巴细胞反应（MLR）中所识别抗原的性质。具体而言，我们希望确定MLR反应性T细胞是否仅识别Ia抗原，或者其特异性是否由非MHC抗原与同基因或同种异体Ia的共同识别所决定。为此，我们使用了11个T细胞杂交瘤，其特征为对Iad具有特异性，并测试了它们对掺入脂质体并结合到玻璃珠表面的分离的I-Ad或I-Ed作出反应的能力。在九个同种异体反应性T细胞杂交瘤（五个I-Ad特异性和四个I-Ed特异性）中，有七个显示对玻璃珠上相关的分离Ia抗原有反应。同样，两个同基因I-Ad特异性T细胞杂交瘤中有两个对玻璃珠上的I-Ad有反应。在两个对玻璃珠上相关Ia抗原无反应的同种异体反应性T细胞杂交瘤中，有一个被证明对胎牛血清（FCS）中的一种抗原具有特异性，该抗原在同种异体Ia抗原（I-Ed）的背景下被识别，因为当使用完整的辅助细胞时，仅在检测培养基中存在FCS或辅助细胞预先用FCS脉冲处理时，才观察到该杂交瘤的反应。评估了FCS抗原和非Ia辅助细胞抗原在刺激九个对玻璃珠上分离的Ia有反应的T细胞杂交瘤中的可能作用。在无血清培养基中生长并进行测试的T细胞杂交瘤仍然能够对珠子上的Ia作出反应。所使用的分离Ia制剂纯度大于90%，并且它们刺激T细胞杂交瘤的能力与非Ia蛋白的污染程度无关。我们从这些研究中得出结论，大多数对携带同种异体或同基因Ia的刺激细胞作出反应的T细胞对Ia抗原本身具有特异性，不需要共同识别其他非Ia抗原；这种识别也不需要Ia抗原加工。
