Department of Pharmacology, Institute of Biosciences, Univ. Estadual Paulista, UNESP, Botucatu, SP, CEP 18618-970, Brazil.
Department of Pharmacology, Institute of Biosciences, Univ. Estadual Paulista, UNESP, Botucatu, SP, CEP 18618-970, Brazil.
J Ethnopharmacol. 2014;151(1):380-5. doi: 10.1016/j.jep.2013.10.056. Epub 2013 Nov 6.
Stem bark and fruit pulp of Hymenaea stigonocarpa Mart ex. Hayne (Fabaceae) has been popularly used to treat inflammation and gastrointestinal diseases including ulcers, diarrhea and gastric pain. The aim of this study was to investigate the intestinal anti-inflammatory activity of a methanol extract derived from the stem bark and diet with fruit pulp of Hymenaea stigonocarpa in the TNBS model of intestinal inflammation in rats.
The intestinal anti-inflammatory activity of stem bark extract (100, 200 and 400mg/kg) and fruit pulp (10% and 5% in diet) was measured against the intestinal inflammatory process induced by TNBS (trinitrobenzesulphonic acid) in rats. The protective effects were evaluated as follows: evaluation of intestinal damage (damage score, extension of lesion, colon weight/length ratio), incidence of diarrhea and adherence to adjacent organs, colon glutathione (GSH) and malondialdehyde (MDA) contents, myeloperoxidase (MPO) and alkaline phosphatase (AP) activities. In addition, in vitro studies on lipid peroxidation in rat brain membranes and phytochemical profile were performed with both stem bark and fruit pulp.
Treatment with 100, 200 and 400mg/kg of stem bark extract and 10% fruit pulp flour showed protective effects in the TNBS-induced colon damage, which was related to inhibition of MPO and AP activities, reduction in colon MDA content, and counteraction of GSH depletion induced by inflammatory process. A concentration-dependent inhibitory effect on the lipid peroxidation in rat brain membranes for stem bark and fruit pulp was determined, with an IC50 value of 5.25 ± 0.23 μg/mL and 27.33 ± 0.09 μg/mL, respectively. Similar phytochemical composition was observed in fruit and stem bark, including mainly flavonoids, condensed tannins and terpenes.
Stem bark extract and fruit pulp flour of Hymenaea stigonocarpa prevented TNBS-induced colonic damage in rats and this protective effect were associated to an improvement of intestinal oxidative stress. The observed anti-inflammatory and antioxidant effects may be associated to the presence of flavonoids and tannins in the stem bark and fruit pulp of Hymenaea stigonocarpa.
Hymenaea stigonocarpa Mart ex. Hayne（豆科）的树皮和果肉已被广泛用于治疗炎症和胃肠道疾病，包括溃疡、腹泻和胃痛。本研究旨在研究来源于 Hymenaea stigonocarpa 树皮的甲醇提取物和果肉饮食在大鼠 TNBS 模型中的肠道抗炎活性。
使用 TNBS（三硝基苯磺酸）诱导大鼠肠道炎症，测定树皮提取物（100、200 和 400mg/kg）和果肉（10%和 5%饮食）的肠道抗炎活性。评估保护作用如下：评估肠道损伤（损伤评分、病变延伸、结肠重量/长度比）、腹泻发生率和与邻近器官的粘连、结肠谷胱甘肽（GSH）和丙二醛（MDA）含量、髓过氧化物酶（MPO）和碱性磷酸酶（AP）活性。此外，对树皮和果肉的大鼠脑组织的脂质过氧化和植物化学特征进行了体外研究。
100、200 和 400mg/kg 的树皮提取物和 10%果肉粉的治疗对 TNBS 诱导的结肠损伤具有保护作用，这与抑制 MPO 和 AP 活性、降低结肠 MDA 含量以及对抗炎症过程中 GSH 耗竭有关。确定了树皮和果肉对大鼠脑组织脂质过氧化的浓度依赖性抑制作用，IC50 值分别为 5.25±0.23μg/mL 和 27.33±0.09μg/mL。果肉和树皮的化学成分相似，主要包括类黄酮、缩合单宁和萜类化合物。
Hymenaea stigonocarpa 的树皮提取物和果肉粉可预防大鼠 TNBS 诱导的结肠损伤，这种保护作用与改善肠道氧化应激有关。观察到的抗炎和抗氧化作用可能与 Hymenaea stigonocarpa 的树皮和果肉中类黄酮和单宁的存在有关。
