Ribeiro Gislane Dos Santos, Martins Diegue Henrique Nascimento, Gomes João Victor Dutra, Davies Noel William, Fagg Christopher William, Simeoni Luiz Alberto, Homem-de-Mello Mauricio, Magalhães Pérola Oliveira, Silveira Dâmaris, Fonseca-Bazzo Yris Maria
Pharmacy Department, Health Sciences School, University of Brasília, Brasilia 70910-900, Brazil.
Central Science Laboratory, University of Tasmania, Hobart, TAS 7005, Australia.
Plants (Basel). 2023 Sep 26;12(19):3393. doi: 10.3390/plants12193393.
We investigated four Cerrado plant species, i.e., (Miers) A.C.Sm, Lam., Gomes, and Mart. ex Hayne, against acetaminophen toxicity using an in vitro assay with HepG2 cells. The activity against acetaminophen toxicity was evaluated using different protocols, i.e., pre-treatment, co-treatment, and post-treatment of the cells with acetaminophen and the plant extracts. HepG2 cell viability after treatment with acetaminophen was 39.61 ± 5.59% of viable cells. In the pre-treatment protocol, the extracts could perform protection with viability ranging from 50.02 ± 15.24% to 78.75 ± 5.61%, approaching the positive control silymarin with 75.83 ± 5.52%. In the post-treatment protocol, all extracts and silymarin failed to reverse the acetaminophen damage. In the co-treatment protocol, the extracts showed protection ranging from 50.92 ± 11.14% to 68.50 ± 9.75%, and silymarin showed 77.87 ± 4.26%, demonstrating that the aqueous extracts of the species also do not increase the toxic effect of acetaminophen. This protection observed in cell viability was accompanied by a decrease in ROS. The extracts' hepatoprotection can be related to antioxidant compounds, such as rutin and mangiferin, identified using HPLC-DAD and UPLC-MS/MS. The extracts were shown to protect HepG2 cells against future APAP toxicity and may be candidates for supplements that could be used to prevent liver damage. In the concomitant treatment using the extracts with APAP, it was demonstrated that the extracts do not present a synergistic toxicity effect, with no occurrence of potentiation of toxicity. The extracts showed considerable cytoprotective effects and important antioxidant characteristics.
我们使用HepG2细胞体外试验，研究了四种塞拉多植物物种，即（米尔斯）A.C.斯迈思、林奈、戈麦斯和马丁·埃克斯·海恩，对抗对乙酰氨基酚毒性的作用。使用不同方案评估了对乙酰氨基酚毒性的活性，即细胞用对乙酰氨基酚和植物提取物进行预处理、共处理和后处理。用对乙酰氨基酚处理后，HepG2细胞活力为存活细胞的39.61±5.59%。在预处理方案中，提取物可发挥保护作用，活力范围为50.02±15.24%至78.75±5.61%，接近阳性对照水飞蓟素的75.83±5.52%。在后处理方案中，所有提取物和水飞蓟素均未能逆转对乙酰氨基酚的损伤。在共处理方案中，提取物显示出50.92±11.14%至68.50±9.75%的保护作用，水飞蓟素显示出77.87±4.26%的保护作用，表明这些物种的水提取物也不会增加对乙酰氨基酚的毒性作用。在细胞活力方面观察到的这种保护作用伴随着活性氧的减少。提取物的肝保护作用可能与通过HPLC-DAD和UPLC-MS/MS鉴定的抗氧化化合物，如芦丁和芒果苷有关。提取物显示出可保护HepG2细胞免受未来对乙酰氨基酚毒性的影响，可能是可用于预防肝损伤的补充剂的候选物。在将提取物与对乙酰氨基酚同时使用的处理中，证明提取物不会产生协同毒性作用，没有毒性增强的情况发生。提取物显示出相当大的细胞保护作用和重要的抗氧化特性。
