Koshino Kurodo, Kanai Nobuo, Yamato Masayuki, Okano Teruo, Yamamoto Masakazu
Kurodo Koshino, Nobuo Kanai, Masakazu Yamamoto, Institute of Gastroenterology, School of Surgery, Tokyo Women's Medical University, Shinjuku-ku, Tokyo 162-8666, Japan.
World J Gastroenterol. 2015 May 7;21(17):5242-9. doi: 10.3748/wjg.v21.i17.5242.
To create a new rat model for drug administration, cell transplantation, and endoscopic examination for the treatment of intestinal diseases.
F344/NJc l-rnu/rnu rats (10-wk-old males, 350-400 g) were used in this study. The rats were anesthetized via 2% isoflurane inhalation. The rat's cecum was isolated from the intestines, and a pouch was created. The remainder of the intestines was rejoined to create an anastomosis. The "side-to-side" anastomosis (SSA) technique initially involves the creation of a 2-cm longitudinal incision into each intestinal wall. To create an anastomosis along the ileal and colonic walls, both intestines were cut, and a continuous suture procedure was performed that included all layers of both intestines. The serous membrane was sutured along the edge and on the anterior wall of the anastomosis. The "end-to-end" anastomosis (EEA) technique was compared with the SSA technique. In the EEA technique, the frontal surfaces of both cut intestinal lumens were joined together by continuous sutures. Additional sutures were made at the serosa. After the anastomotic intestine was successfully constructed, the two intestinal lumens that were cut at the isolated cecum were managed. In addition, one luminal side of the pouch remained open to create an artificial anus on the dorsum as a passage for the residual substances in the pouch. Finally, small animal endoscopy was used to observe the inside of the pouch.
In this animal model, mucus and feces are excreted through the reconstructed passage. Accordingly, the cecal pouch mucosa was not obstructed or contaminated by feces, thus facilitating observations of the luminal surface of the intestine. The endoscopic observation of the cecal pouch provided clear visualization given the absence of feces. The membrane surface of the cecum was clearly observed. Two methods of creating an anastomotic intestine, the "SSA" and "EEA" techniques, were compared with regard to animal survival rate, complication rate, and operation time. The SSA technique resulted in a significantly increased survival rate and a lower incidence of complications in rat models compared with the EEA technique. The complications of stenosis and leakage resulted in death in the EEA technique. Thus, the EEA technique exhibited a lower survival rate compared with the SSA technique. However, the SSA technique required a significantly longer operation time compared with the EEA technique.
Our new rat model is potentially useful for the development of a novel treatment for intestinal diseases.
创建一种新的大鼠模型，用于药物给药、细胞移植及内镜检查，以治疗肠道疾病。
本研究使用F344/NJc l-rnu/rnu大鼠（10周龄雄性，体重350 - 400克）。通过吸入2%异氟醚对大鼠进行麻醉。将大鼠的盲肠与肠道分离并制作一个袋状结构。将肠道的其余部分重新连接以形成吻合口。“侧对侧”吻合术（SSA）最初是在每个肠壁上做一个2厘米的纵向切口。为了在回肠和结肠壁之间形成吻合口，将两段肠道切断，并进行连续缝合，包括两层肠道的所有层次。沿着吻合口边缘和前壁缝合浆膜。将“端对端”吻合术（EEA）与SSA技术进行比较。在EEA技术中，将两个切断的肠腔正面通过连续缝合连接在一起。在浆膜处进行额外缝合。在成功构建吻合肠段后，处理在分离的盲肠处切断的两个肠腔。此外，袋状结构的一个腔侧保持开放，在背部形成一个人工肛门作为袋内残留物质的通道。最后，使用小动物内镜观察袋状结构内部。
在这个动物模型中，黏液和粪便通过重建通道排出。因此，盲肠袋黏膜未被粪便阻塞或污染，便于观察肠腔表面。由于没有粪便，对盲肠袋的内镜观察提供了清晰的视野。清晰观察到盲肠的膜表面。比较了两种创建吻合肠段的方法，即“SSA”和“EEA”技术在动物存活率、并发症发生率和手术时间方面的差异。与EEA技术相比，SSA技术在大鼠模型中导致存活率显著提高，并发症发生率降低。EEA技术中的狭窄和渗漏并发症导致大鼠死亡。因此，与SSA技术相比，EEA技术的存活率较低。然而，与EEA技术相比，SSA技术所需的手术时间显著更长。
我们的新大鼠模型可能对开发新型肠道疾病治疗方法有用。
