Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke University, Leipziger Str. 44, Magdeburg 39120, Germany.
Cancers (Basel). 2011 Aug 8;3(3):3156-68. doi: 10.3390/cancers3033156.
Leukotrienes (LT) mediate allergic and inflammatory processes. Previously, we identified significant changes in the expression pattern of LT receptors in the gastric mucosa after eradication of Helicobacter pylori infection. The aim of the present study was to evaluate the expression of 5-lipoxygenase (5-LOX) and LT receptors in gastric cancer (GC).
The expression of 5-LOX and receptors for LTB4 (BLT-1, BLT-2) and cysteinyl-LT (CysLT-1, CysLT-2) were analyzed by immunohistochemistry (IHC) in GC samples of 35 consecutive patients who underwent gastrectomy and in 29 tumor-free tissue specimens from gastric mucosa.
Male-to-female ratio was 24:11. The median age was 70 years (range 34-91). Twenty-two patients had GC of intestinal, six of diffuse, six of mixed and one of undifferentiated type. The IHC analysis showed a nearly ubiquitous expression of studied proteins in GC (88-97%) and in tumor-free specimens as well (89-100%). An increase in the immunoreactive score of both BLT receptors and CysLT-1 was observed in GC compared to tumor-free gastric mucosa (p < 0.001 for BLT-1; p < 0.01 for BLT-2 and CysLT-1, Mann-Whitney U-test). No differences in the IHC expression of 5-LOX and CsyLT-2 were observed between GC and tumor-free mucosa. The expression of BLT-2, CysLT-1 and CysLT-2 was increased in GC of intestinal type when compared to the diffuse type (p < 0.05; Mann-Whitney U-test).
LTB4 receptors and CysLT-1 are up-regulated in GC tissue implying a role in gastric carcinogenesis.
白三烯（LT）介导过敏和炎症过程。此前，我们发现幽门螺杆菌感染根除后胃黏膜中 LT 受体的表达模式发生了显著变化。本研究旨在评估 5-脂氧合酶（5-LOX）和 LT 受体在胃癌（GC）中的表达。
采用免疫组织化学（IHC）分析 35 例连续接受胃切除术的 GC 患者和 29 例胃黏膜无肿瘤组织标本中 5-LOX 和 LTB4 受体（BLT-1、BLT-2）和半胱氨酰-LT（CysLT-1、CysLT-2）的表达。
男女比例为 24:11。中位年龄为 70 岁（范围 34-91）。22 例患者为肠型 GC，6 例弥漫型，6 例混合性，1 例未分化型。IHC 分析显示，研究蛋白在 GC（88-97%）和无肿瘤标本中均有几乎普遍的表达（89-100%）。与无肿瘤胃黏膜相比，GC 中 BLT 受体和 CysLT-1 的免疫反应性评分均升高（BLT-1 为 p < 0.001；BLT-2 和 CysLT-1 为 p < 0.01，Mann-Whitney U 检验）。GC 与无肿瘤黏膜之间 5-LOX 和 CsyLT-2 的 IHC 表达无差异。与弥漫型相比，肠型 GC 中 BLT-2、CysLT-1 和 CysLT-2 的表达增加（p < 0.05；Mann-Whitney U 检验）。
LTB4 受体和 CysLT-1 在 GC 组织中上调，提示其在胃癌发生中起作用。
