Institute of Forest Genetics, Pacific Southwest Forest and Range Experiment Station, Forest Service, U.S. Department of Agriculture, Berkeley, CA 94701.
Proc Natl Acad Sci U S A. 1989 Dec;86(23):9347-9. doi: 10.1073/pnas.86.23.9347.
Restriction fragment length polymorphisms in controlled crosses were used to infer the mode of inheritance of chloroplast DNA and mitochondrial DNA in coast redwood (Sequoia sempervirens D. Don Endl.). Chloroplast DNA was paternally inherited, as is true for all other conifers studied thus far. Surprisingly, a restriction fragment length polymorphism detected by a mitochondrial probe was paternally inherited as well. This polymorphism could not be detected in hybridizations with chloroplast probes covering the entire chloroplast genome, thus providing evidence that the mitochondrial probe had not hybridized to chloroplast DNA on the blot. We conclude that mitochondrial DNA is paternally inherited in coast redwood. To our knowledge, paternal inheritance of mitochondrial DNA in sexual crosses of a multicellular eukaryotic organism has not been previously reported.
利用控制杂交中的限制性片段长度多态性，推断出海岸红杉（SequoiasempervirensD.DonEndl.）叶绿体 DNA 和线粒体 DNA 的遗传方式。与迄今为止研究的所有其他针叶树一样，叶绿体 DNA 是父系遗传的。令人惊讶的是，线粒体探针检测到的限制性片段长度多态性也是父系遗传的。在用覆盖整个叶绿体基因组的叶绿体探针杂交中，无法检测到这种多态性，这为线粒体探针没有与印迹上的叶绿体 DNA 杂交提供了证据。我们得出结论，线粒体 DNA 在海岸红杉的有性杂交中是父系遗传的。据我们所知，在多细胞真核生物的有性杂交中，线粒体 DNA 的父系遗传以前没有报道过。
