Department of Horticultural Science, University of Guelph, N1G 2W1, Guelph, Ont., Canada.
Plant Cell Rep. 1992 Apr;11(3):163-8. doi: 10.1007/BF00232172.
A rapid, one-step procedure has been developed for inducing direct organogenesis and somatic embryogenesis in cultures of Phaseolus coccineus L., P. acutifolius A., P. aureus L. [Vigna radiata L. Wilczek] and P. wrightii L. Development of somatic embryos and shoot buds occurred within 6-8 weeks of culture from intact seedlings raised on MS (Murashige and Skoog 1962) medium supplemented with N(6)-benzylaminopurine (BAP). Shoot buds or embryoids originated from subepidermal tissue of the regions adjacent to the shoot apex, hypocotyl and cotyledonary axils. While P. acutifolius and P. aureus were regenerated via shoot formation and P. wrightii by somatic embryogenesis, both embryogenesis and shoot regeneration were observed in P. coccineus. Relatively higher levels of BAP, 50-80 μM, were found to be optimal for inducing regeneration while lower concentrations were ineffective. About 40-70 shoots and 70-250 somatic embryos were produced per responding seedling. Regenerated shoots and somatic embryos developed into whole plants on a basal medium or the one supplemented with 1 μM naphthaleneacetic acid.
已开发出一种快速、一步的程序，可在 Phaseolus coccineus L.、P. acutifolius A.、P. aureus L. [Vigna radiata L. Wilczek] 和 P. wrightii L. 的培养物中诱导直接器官发生和体细胞胚胎发生。从在补充有 N(6)-苄基氨基嘌呤 (BAP) 的 MS（Murashige 和 Skoog 1962）培养基上培养的完整幼苗中，在 6-8 周的培养时间内即可发育出体细胞胚和芽。芽或胚状体起源于靠近芽尖、下胚轴和子叶腋的表皮组织。虽然 P. acutifolius 和 P. aureus 通过芽形成再生，而 P. wrightii 通过体细胞胚胎发生再生，但 P. coccineus 同时观察到胚胎发生和芽再生。发现 50-80 μM 的相对较高浓度的 BAP 最适合诱导再生，而较低浓度则无效。每个反应的幼苗可产生约 40-70 个芽和 70-250 个体细胞胚。再生的芽和体细胞胚在基础培养基或补充有 1 μM 萘乙酸的培养基上发育成完整的植株。
