USDA-ARS Crops and Entomology Research Unit, P.O. Box 1633, 48823-6633, East Lansing, MI, USA.
Plant Cell Rep. 1987 Feb;6(1):46-9. doi: 10.1007/BF00269737.
Roots, callus and/or globular structures were produced on primary leaf and distal cotyledon explants of pinto bean (Phaseolus vulgaris L. cv. UI 114) cultured on semisolid MS medium with a wide range of 2,4-D concentrations (0.01 to 80 mg/L) with either 0 or 1.0 mg/L kinetin. Explants rooted at lower 2,4-D concentrations than at those favoring globule formation on callus, although roots, callus and globules often developed from the same explant. Isolated opaque green globular structures developed when callus initiated on media with 3 or more mg/L 2,4-D was subcultured in liquid MS + 30 mg/L 2,4-D. These structures multiplied with a fresh weight doubling time of 8-9 days in MS + 30 mg/L 2,4-D. Although this multiplicative behavior and opaque color were reminiscent of embryoids reported for other species, no cotyledons or roots were seen.
在半固态 MS 培养基上培养的菜豆（Phaseolus vulgaris L. cv. UI 114）的初级叶片和远轴子叶外植体上产生了根、愈伤组织和/或球状结构，2,4-D 浓度范围很广（0.01 至 80 mg/L），有或没有 1.0 mg/L 的激动素。与有利于愈伤组织形成球状结构的浓度相比，外植体在较低的 2,4-D 浓度下生根，尽管根、愈伤组织和球状结构通常都来自同一外植体。当在含有 3 毫克/升或更多 2,4-D 的培养基上起始愈伤组织时，将其继代培养在液体 MS+30 毫克/升 2,4-D 中，会形成分离的不透明绿色球状结构。这些结构在 MS+30 毫克/升 2,4-D 中以 8-9 天的新鲜重量倍增时间倍增。尽管这种增殖行为和不透明颜色使人联想到其他物种报告的胚状体，但没有看到子叶或根。
