Department of Cell and Molecular Pharmacology, Medical University of South Carolina, 171 Ashley Avenue, 29425, Charleston, SC.
J Am Soc Mass Spectrom. 1995 Apr;6(4):257-63. doi: 10.1016/1044-0305(95)00025-9.
A new in-line surface-induced dissociation device has been designed and characterized in a high performance four-sector tandem mass spectrometer. The design incorporates a target electrode parallel to the ion beam axis and an angled deflector plate (45° relative to the ion beam) to provide large collision angles. In addition, an extraction electrode (parallel to the target electrode) is employed to efficiently extract product ions from the target surface. Results obtained with this device indicate high internal energy deposition (up to 16. 3 eV) as measured with the thermometer ions W(CO) 6 (+·) and Si(C2H5)4/+·, as evidenced by extensive dissociation of the refractory pyrene molecular ion, and as indicated by the b 3/y 2 ratio in the product ion spectrum of leucine enkephalin. High resolution provided by the four-sector instrument for both precursor ions and product ions allows the observation of previously unobserved dissociation products in the surface-induced dissociation spectra of Si(C2H5)4/+· and novel ion-surface reaction products in spectra of W(CO)6/+· ions after collisions with hydrocarbon-covered surfaces. Both hydrogen atom and hydrocarbon abstraction products are observed. The dissociation efficiencies measured with the in-line device are approximately 1% when hydrocarbon-coated surfaces are used and increase fivefold with a fluorinated surface.
一种新的在线表面诱导解离装置已被设计并在高性能四极串联质谱仪中进行了表征。该设计采用了一个与离子束轴平行的靶电极和一个倾斜的偏转板（相对于离子束成 45°角），以提供较大的碰撞角度。此外，还采用了一个萃取电极（与靶电极平行），以有效地从靶表面提取产物离子。该装置的结果表明，内部能量沉积很高（高达 16.3 eV），这可以通过温度计离子 W(CO)6 (+·)和 Si(C2H5)4/+·来测量，其证据是难熔的苝分子离子的广泛解离，以及亮氨酸脑啡肽产物离子谱中的 b 3/y 2 比值所表明的。四极仪器为前体离子和产物离子提供的高分辨率允许观察到 Si(C2H5)4/+·的表面诱导解离谱中以前未观察到的解离产物，以及 W(CO)6/+·离子与烃覆盖表面碰撞后的新型离子-表面反应产物。观察到了氢原子和碳氢化合物的抽取产物。当使用烃涂覆表面时，在线装置测量的解离效率约为 1%，而使用氟化表面时则增加了五倍。
