Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China.
Bioresour Technol. 2014 Jan;151:120-7. doi: 10.1016/j.biortech.2013.10.031. Epub 2013 Oct 18.
The effect of osmotic pressure on erythritol and mannitol production by an osmophilic yeast strain of Yarrowia lipolytica CICC 1675 using glycerol as the sole carbon source was investigated. Appropriately high osmotic pressure was found to enhance erythritol production and inhibit mannitol formation. A novel two-stage osmotic pressure control fed-batch strategy based on the kinetic analysis was developed for higher erythritol yield and productivity. During the first 96 h, the osmotic pressure was maintained at 4.25 osmol/kg by feeding glycerol to reduce the inhibition of cell growth. After 132 h, the osmotic pressure was controlled at 4.94 osmol/kg to maintain a high dp(ery)/dt. Maximum erythritol yield of 194.3g/L was obtained with 0.95 g/L/h productivity, which were 25.7% and 2.2%, respectively, improvement over the best results in one-stage fed-batch fermentation. This is the first report that a novel osmotic pressure control fed-batch strategy significantly enhanced erythritol production.
用甘油作为唯一碳源，研究了高渗透压对嗜脂酵母假丝酵母 CICC 1675 菌株合成赤藓糖醇和甘露醇的影响。发现适当高的渗透压可以促进赤藓糖醇的生产，抑制甘露醇的形成。根据动力学分析，提出了一种新型的两阶段渗透压控制补料分批策略，以获得更高的赤藓糖醇产率和生产力。在最初的 96 h 内，通过添加甘油来维持 4.25 osmol/kg 的渗透压，以降低细胞生长的抑制作用。132 h 后，将渗透压控制在 4.94 osmol/kg，以保持高 dp(ery)/dt。最大赤藓糖醇产量为 194.3 g/L，产率为 0.95 g/L/h，与单阶段补料分批发酵的最佳结果相比，分别提高了 25.7%和 2.2%。这是首次报道一种新型渗透压控制补料分批策略显著提高了赤藓糖醇的产量。
