Koyanagi Y, Harada S, Yamamoto N
Cancer Lett. 1986 Mar;30(3):299-310. doi: 10.1016/0304-3835(86)90054-6.
A cell culture system was developed for the continuous and efficient production of acquired immune deficiency syndrome (AIDS) retrovirus. After infection of a human T-cell line Molt-4 with HTLV-III and LAV the cells grow permanently and produce large amounts of virus continuously. The yields of production of virus were assessed either with reverse transcriptase activity or a newly established biological quantitation assay of active virus. The amounts of virus with this cell system were much higher than those of the H9 cell system. This procedure enabled us first to compare the two viral isolates HTLV-III and LAV directly in the same cell line. Establishment of the culture system, allowing efficient production of AIDS retroviruses, provides a useful tool for the isolation of the virus from patients with AIDS and for more basic research, such as the mechanisms of immune destruction caused by the virus leading to the occurrence of various malignancies.
开发了一种细胞培养系统，用于持续高效地生产获得性免疫缺陷综合征（艾滋病）逆转录病毒。用人T细胞系Molt-4感染HTLV-III和LAV后，细胞持续生长并持续产生大量病毒。通过逆转录酶活性或新建立的活性病毒生物学定量测定法评估病毒的产量。该细胞系统产生的病毒量远高于H9细胞系统。此方法使我们首次能够在同一细胞系中直接比较两种病毒分离株HTLV-III和LAV。该培养系统的建立可高效生产艾滋病逆转录病毒，为从艾滋病患者中分离病毒以及进行更多基础研究（如病毒导致各种恶性肿瘤发生的免疫破坏机制）提供了有用的工具。
