Thompson T C, Chung L W
Endocrinology. 1986 Jun;118(6):2437-44. doi: 10.1210/endo-118-6-2437.
Enlargement of the chimeric ventral prostate gland (VP) was induced by directly implanting either fetal urogenital sinus mesenchyme (UGM) or intact fetal urogenital sinus (UGS) into the VP of intact adult rats. The macromolecular content in the chimeric prostate increased from 40-100% (UGM implants) to 200-300% (UGS implants) above control levels. The enlargement of the prostate gland was the result of growth from both the donor tissue and the host gland. Growth of the donor fetal UGS within the host prostate gland may account for the difference observed between the growth induced by fetal UGS and fetal UGM implants. Because fetal UGM regressed when implanted and grown under the renal capsules, the observation of growth in the adult rat VP induced by fetal UGM, either by implanting UGM in situ or forming tissue recombinants of UGM and the adult VP, suggests that fetal UGM requires close association with the VP for the induction of growth to occur. The concentration of an epithelial androgen-dependent protein, the prostatic binding protein (PBP), expressed by the enlarged lobe of the rat VP was similar to that of the control lobe of rat VP. The adult host gland, rather than donor implants, appeared to determine the levels of expression of PBP within the chimeric prostate gland. Immunofluorescence data indicated that PBP was distributed evenly throughout most of the prostatic acini. PBP also accumulated in the lumen of the prostatic acini. Positive immunofluorescence, although less intense, was detected in the UGS remnant, suggesting that fetal UGS was induced by the intact adult VP environment to express PBP. We observed a developmental restriction in the ability of donor prostatic tissues to induce enlargement of the host prostate gland. Fetal UGS was the most effective inducer, whereas neonatal prostatic tissue was marginally effective, and adult prostatic tissue or stromal cells derived from adult VP were completely ineffective.
通过将胎儿泌尿生殖窦间充质（UGM）或完整的胎儿泌尿生殖窦（UGS）直接植入成年大鼠完整的腹侧前列腺（VP），可诱导嵌合腹侧前列腺增大。嵌合前列腺中的大分子含量比对照水平增加了40 - 100%（UGM植入）至200 - 300%（UGS植入）。前列腺的增大是供体组织和宿主腺体共同生长的结果。宿主前列腺内供体胎儿UGS的生长可能解释了胎儿UGS和胎儿UGM植入诱导生长之间观察到的差异。由于胎儿UGM植入并在肾被膜下生长时会退化，胎儿UGM通过原位植入UGM或形成UGM与成年VP的组织重组体诱导成年大鼠VP生长的观察结果表明，胎儿UGM需要与VP紧密关联才能诱导生长发生。大鼠VP增大叶表达的上皮雄激素依赖性蛋白——前列腺结合蛋白（PBP）的浓度与大鼠VP对照叶相似。成年宿主腺体而非供体植入物似乎决定了嵌合前列腺内PBP的表达水平。免疫荧光数据表明，PBP均匀分布在大多数前列腺腺泡中。PBP也在前列腺腺泡腔内积聚。在UGS残余物中检测到阳性免疫荧光，尽管强度较弱，这表明完整的成年VP环境诱导胎儿UGS表达PBP。我们观察到供体前列腺组织诱导宿主前列腺增大的能力存在发育限制。胎儿UGS是最有效的诱导剂，而新生前列腺组织效果微弱，成年前列腺组织或源自成年VP的基质细胞则完全无效。
