Chung L W, Cunha G R
Prostate. 1983;4(5):503-11. doi: 10.1002/pros.2990040509.
Embryonic urogenital sinus mesenchyme (UGM) has been demonstrated previously to be a potent inductor of prostatic morphogenesis and functional differentiation when associated with either embryonic urogenital sinus epithelium (UGE) or urothelium derived from adult urinary bladder (ABLE) and grown in male hosts. To determine the role of mesenchyme in prostatic acinar growth, homotypic tissue recombinants of 16-day-old embryonic mouse urogenital sinus mesenchyme and epithelium (UGM mouse + UGE mouse) were prepared in which the relative amounts of UGM to UGE were varied from approximately 0.1:1 to 100:1. Recombinants were grown for 1 month in intact male hosts after which the amount of acinar growth was assessed by histological analysis and by determination of wet weight and DNA content. The latter criteria are valid measures of acinar content of histologically normal prostatic tissue because the rodent prostate is composed of greater than 80% ductal-acinar tissue. From this analysis the amount of acinar growth was found to be determined by the amount of mesenchymal tissue and was independent of the amount of epithelium utilized to prepare the tissue recombinants. Similarly, the magnitude of growth in hetero-specific rat-mouse tissue recombinants prepared with urogenital sinus epithelium or adult bladder epithelium (ABLE) and urogenital sinus mesenchyme (UGM mouse + UGE rat, UGM mouse + ABLE rat, UGM rat + UGE mouse, or UGM rat + ABLE mouse) is determined by the source of UGM. Although the initial DNA content per UGM is comparable between mouse and rat, the overall acinar growth induced by UGM rat is about 10-fold greater than that induced by UGM mouse when recombined reciprocally with UGE mouse and UGE rat, respectively. These results suggest that UGM is of fundamental importance as a regulator of prostatic epithelial growth. The relevance of this finding to the development of human benign prostatic hyperplasia is discussed.
胚胎泌尿生殖窦间充质（UGM）先前已被证明，当与胚胎泌尿生殖窦上皮（UGE）或成年膀胱来源的尿路上皮（ABLE）相关联并在雄性宿主中生长时，它是前列腺形态发生和功能分化的有效诱导物。为了确定间充质在前列腺腺泡生长中的作用，制备了16日龄胚胎小鼠泌尿生殖窦间充质和上皮的同型组织重组体（UGM小鼠+UGE小鼠），其中UGM与UGE的相对量从约0.1:1变化到100:1。重组体在完整的雄性宿主中生长1个月，之后通过组织学分析以及测定湿重和DNA含量来评估腺泡生长量。后两个标准是组织学正常前列腺组织腺泡含量的有效测量指标，因为啮齿动物前列腺由超过80%的导管腺泡组织组成。通过该分析发现，腺泡生长量由间充质组织的量决定，并且与用于制备组织重组体的上皮量无关。同样，用泌尿生殖窦上皮或成年膀胱上皮（ABLE）与泌尿生殖窦间充质制备的异种大鼠 - 小鼠组织重组体（UGM小鼠+UGE大鼠、UGM小鼠+ABLE大鼠、UGM大鼠+UGE小鼠或UGM大鼠+ABLE小鼠）的生长幅度由UGM的来源决定。尽管每单位UGM的初始DNA含量在小鼠和大鼠之间相当，但当分别与UGE小鼠和UGE大鼠相互重组时，UGM大鼠诱导的总体腺泡生长比UGM小鼠诱导的大约大10倍。这些结果表明，UGM作为前列腺上皮生长的调节因子至关重要。本文讨论了这一发现与人类良性前列腺增生发展的相关性。
