Kim Inyoung, Lee Kyeong-Ryeol, Park Mid-Eum, Kim Hyun Uk
Department of Molecular Biology Sejong University Seoul Republic of Korea.
Department of Agricultural Biotechnology, National Institute of Agricultural Sciences Rural Development Administration Jeonju Republic of Korea.
Plant Direct. 2022 Apr 3;6(4):e395. doi: 10.1002/pld3.395. eCollection 2022 Apr.
Triacylglycerol (TAG), an ester derived from glycerol and three fatty acids (FAs), is synthesized during seed development and controlled by transcriptional regulation. We examined the mechanism regulating the FA composition of developing seeds. The seed-specific () transcription factor was upregulated by (). DPBF2 showed transcriptional activity in yeast and localized to the nucleus in Arabidopsis protoplast cells. The Arabidopsis homozygous T-DNA mutant and transgenic lines overexpressing of 2 using a seed-specific phaseolin promoter in wild-type (WT) Arabidopsis and in showed similar FA composition profiles in their seeds. Their 18:2 and 20:1 FA contents were higher, but 18:1 and 18:3 contents were lower than that of WT. Transcript levels of (), , (), , (), and () are increased in -overexpressing seeds. Besides, and were upregulated by DPBF2, LEC1-LIKE (L1L), and NUCLEAR FACTOR-YC2 (NF-YC2) transcriptional complex based on tobacco protoplast transcriptional activation assay. These results suggest that DPBF2 effectively modulates the expression of genes encoding FA desaturases, elongase, and acyl-editing enzymes for modifying the unsaturated FA composition in seeds.
三酰甘油（TAG）是一种由甘油和三种脂肪酸（FAs）衍生而来的酯，在种子发育过程中合成并受转录调控。我们研究了调控发育中种子脂肪酸组成的机制。种子特异性（）转录因子被（）上调。DPBF2在酵母中表现出转录活性，并定位于拟南芥原生质体细胞的细胞核中。拟南芥纯合T-DNA突变体以及在野生型（WT）拟南芥和（）中使用种子特异性菜豆蛋白启动子过表达2的转基因系，其种子中的脂肪酸组成谱相似。它们的18:2和20:1脂肪酸含量较高，但18:1和18:3含量低于野生型。在过表达（）的种子中，（）、（）、（）、（）、（）和（）的转录水平增加。此外，基于烟草原生质体转录激活试验，（）和（）被DPBF2、类LEC1（L1L）和核因子YC2（NF-YC2）转录复合体上调。这些结果表明，DPBF2有效地调节了编码脂肪酸去饱和酶、延长酶和酰基编辑酶的基因的表达，以改变种子中不饱和脂肪酸的组成。
