Agricultural Research Service, United States Department of Agriculture, Department of Plant Pathology, University of Wisconsin, 53706, Madison, WI.
Theor Appl Genet. 1990 Oct;80(4):545-51. doi: 10.1007/BF00226758.
Restriction fragment length polymorphism (RFLP) markers were used to distinguish the chromosomes of Solanum brevidens from those of potato (S. tuberosum) in a fertile somatic hybrid. The hybrid had markers that account for all 24 chromosome arms from each parent, indicating that the hybrid contained at least one copy of each chromosome from each parent. The markers were then used to follow segregation of chromosomes in sexual progeny that resulted from a cross of the somatic hybrid with the potato cultivar 'Katahdin'. Approximately 10% of the sexual progeny lacked one or more of the markers specific to S. brevidens. No one chromosome or marker appeared to be lost preferentially. This infrequent absence of a chromosome marker derived from the wild parent could be explained by intergenomic pairing and recombination. The loss of a marker band for chromosome 8, coupled with the retention of two flanking markers, suggested that a small region of DNA was deleted during regeneration of the somatic hybrid. These results show the value of RFLP analysis when applied to somatic hybrids and their progeny. Clearly, RFLPs will be useful for following the DNA from wild species during its introgression into potato cultivars.
限制片段长度多态性（RFLP）标记被用来区分可育体细胞杂种中短日照茄（Solanum brevidens）和马铃薯（S. tuberosum）的染色体。杂种具有来自每个亲本的所有 24 条染色体臂的标记，表明杂种至少包含每个亲本的每条染色体的一个副本。然后，这些标记被用来跟踪体细胞杂种与马铃薯品种‘Katahdin’杂交产生的有性后代中染色体的分离。大约 10%的有性后代缺乏一个或多个特定于短日照茄的标记。没有一条或多个染色体标记优先丢失。这种来自野生亲本的染色体标记罕见缺失可以通过基因组间配对和重组来解释。8 号染色体标记带的丢失，加上两个侧翼标记的保留，表明在体细胞杂种的再生过程中，一小段 DNA 被删除。这些结果表明 RFLP 分析在体细胞杂种及其后代中的应用价值。显然，RFLP 将有助于在野生种的 DNA 导入马铃薯品种的过程中对其进行跟踪。
