Institute of Forest Genetics and Plant Physiology, The Swedish University of Agricultural Sciences, S-901 83, Umeå, Sweden.
Theor Appl Genet. 1990 Nov;80(5):641-7. doi: 10.1007/BF00224224.
Restriction fragment analysis and heterologous hybridization of chloroplast (cp) DNA was used to develop species-specific markers for P. tabulaeformis, P. yunnanensis and P. massoniana. Fragment patterns created by the BclI and DraI restriction enzymes and hybridization patterns to the psbC and psbD probes were distinctive among the three species. No intraspecific variation was detected with respect to any of the cpDNA markers developed in this study. The cpDNA markers obtained were subsequently used to examine the parentage of P. densata, a putative Tertiary hybrid between P. tabulaeformis and P. yunnanensis. The analysis demonstrated for the first time that P. densata populations accommodate chloroplast genomes of P. tabulaeformis and P. yunnanensis, which strongly supports earlier suggestions of the hybrid origin of this species. It appears that P. densata represents a stabilized natural hybrid that has become adapted to high mountain environments where neither of the parental species can normally grow.
采用叶绿体（cp）DNA 的限制性片段分析和异源杂交技术，为白皮松、云南松和马尾松开发了种特异性标记。BclI 和 DraI 两种限制性内切酶的酶切图谱以及 psbC 和 psbD 探针的杂交图谱在这三个物种中具有明显的特征。在本研究中开发的任何 cpDNA 标记物中，都未检测到种内变异。随后，将获得的 cpDNA 标记物用于研究疑似由白皮松和云南松形成的第三纪杂种华山松的亲子关系。分析首次表明，华山松种群容纳了白皮松和云南松的叶绿体基因组，这强烈支持了该物种杂种起源的早期假说。看来，华山松是一种稳定的自然杂种，已经适应了高山环境，而其亲本种通常无法在这些环境中生长。
