Department of Forestry, Michigan State University, 48824, East Lansing, MI, USA.
Theor Appl Genet. 1989 Dec;78(6):768-74. doi: 10.1007/BF00266656.
Chloroplast DNA (cpDNA) was purified from blue spruce (Picea pungens Engelm.) and white spruce [P. glauca (Moench) Voss], and was digested with several different restriction endonucleases. Restriction fragment length polymorphisms (RFLPs) were identified that differentiated the cpDNA of both species. Intraspecific conservation of the RFLPs that differentiated each species was confirmed by examining trees from across the natural range of each species. Ten F1 hybrids were examined, and the cpDNA from each showed the banding pattern of the paternal species. Cloned Petunia cpDNA containing part of the rbcL gene hybridized to polymorphic bands, while a cloned maize mtDNA probe of the coxII gene failed to hybridize to any band.
叶绿体 DNA（cpDNA）从蓝云杉（Picea pungens Engelm.）和白云杉（P. glauca（Moench）Voss）中纯化出来，并使用几种不同的限制内切酶进行消化。鉴定出了区分这两个物种的 cpDNA 的限制片段长度多态性（RFLP）。通过检查每个物种自然分布范围内的树木，证实了区分每个物种的 RFLP 在种内是保守的。检查了 10 个 F1 杂种，每个杂种的 cpDNA 都显示出父本物种的带型。含有部分 rbcL 基因的克隆矮牵牛 cpDNA 与多态性带杂交，而克隆玉米 mtDNA 探针 coxII 基因未能与任何带杂交。
