红杉、花旗松、翠柏和火炬松叶绿体 DNA 限制片段长度多态性

Chloroplast DNA restriction fragment length polymorphism in Sequoia sempervirens D. Don Endl., Pseudotsuga menziesii (Mirb.) Franco, Calocedrus decurrens (Torr.), and Pinus taeda L.

机构信息

U.S. Department of Agriculture, Institute of Forest Genetics, Pacific Southwest Forest and Range Experiment Station, Forest Service, Box 245, 94701, Berkeley, CA, USA.

出版信息

Theor Appl Genet. 1991 Jan;81(1):83-9. doi: 10.1007/BF00226116.

DOI:10.1007/BF00226116

PMID:24221163

Abstract

The extent and type of chloroplast DNA restriction fragment length polymorphism was determined among individual tree samples of coast redwood, Douglas fir, incense-cedar, and loblolly pine. A total of 107 trees was surveyed for three restriction enzymes (BamHI, EcoRI, HindIII) and six chloroplast DNA probes from petunia (P3, P4, P6, P8, P10, S8). The probes comprise 64% of the petunia chloroplast genome. Polymorphisms were detected in all species but loblolly pine. Coast redwood and incense-cedar had a small number of rare variants, whereas Douglas fir had one highly polymorphic region of insertions/deletions in sequences revealed by the P6 probe from petunia. The mutation hotspot is currently being studied by DNA sequence analysis.

摘要

对海岸红杉、花旗松、香柏和火炬松的个体树木样本进行了叶绿体 DNA 限制酶切片段长度多态性的研究。使用三种限制酶（BamHI、EcoRI、HindIII）和来自矮牵牛的六个叶绿体 DNA 探针（P3、P4、P6、P8、P10、S8）对 107 棵树进行了调查。这些探针占矮牵牛叶绿体基因组的 64%。除了火炬松外，其他所有物种都检测到了多态性。海岸红杉和香柏的稀有变体数量较少，而花旗松则在 P6 探针揭示的序列中存在一个高度多态的插入/缺失区域。该突变热点目前正在通过 DNA 序列分析进行研究。