Department of Environmental Sciences, University of Warwick, CV4 7AL, Coventry, UK.
Microb Ecol. 1983 Oct;9(3):215-26. doi: 10.1007/BF02097738.
The uptake kinetics of leucine and the assimilation and respiration of leucine, glycine, glutamate and arginine by a marinePseudomonas sp. was evaluated to determine whether the uptake and efficiency of substrate utilization of free-living bacteria differed from that of bacteria associated with surfaces. Bacteria were allowed to attach to plastic substrata with known hydrophilicities, as measured by advancing water contact angle (θ A); these were Thermanox, poly(vinylidene fluoride), poly(ethelene) and poly(tetrafluoroethylene). The assimilation and respiration of surface-associated bacteria depended on the amino acid and substratumθ A, but assimilation by surface-associated cells was generally greater than and respiration was generally less than that by free-living bacteria. The uptake kinetics with leucine demonstrated that the half saturation constant (K) of surface-associated bacteria was greater than that for free-living cells. The Vmax values for surface-associated and free-living bacteria were similar, except for cells associated with poly(tetrafluoroethylene), which had a higher Vmax value.
我们评估了海洋假单胞菌对亮氨酸的摄取动力学以及对亮氨酸、甘氨酸、谷氨酸和精氨酸的同化和呼吸作用，以确定游离细菌的底物利用摄取和效率是否与附着在表面的细菌不同。让细菌附着在亲水性已知的塑料基质上，用前进水接触角（θA）来测量；这些基质分别是 Thermanox、聚偏二氟乙烯、聚乙烯和聚四氟乙烯。表面附着细菌的同化和呼吸作用取决于氨基酸和基质θA，但表面附着细胞的同化作用通常大于游离细菌，而呼吸作用通常小于游离细菌。亮氨酸的摄取动力学表明，表面附着细菌的半饱和常数（K）大于游离细胞。表面附着和游离细菌的 Vmax 值相似，除了与聚四氟乙烯相关的细胞，其 Vmax 值较高。
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