Ellwood D C, Keevil C W, Marsh P D, Brown C M, Wardell J N
Philos Trans R Soc Lond B Biol Sci. 1982 Jun 11;297(1088):517-32. doi: 10.1098/rstb.1982.0058.
In natural ecosystems, microbial activity is often associated with the presence of a surface, particularly in low-nutrient environments. The chemostat allows the study of such low-nutrient environments together with the precise control of other growth parameters. By using this system, enrichment cultures with inocula from two different river sources have been made. A more diverse community attached itself to surfaces placed in the chemostat when the cultures were carbon-limited than when the limiting nutrient was nitrogen. Further studies on a pseudomonad isolated from the carbon-limited enrichment cultures have shown that surface-associated organisms grow at approximately twice the rate of the same organism in the free surrounding medium. A hypothesis to explain this phenomenon based on the chemiosmotic theory is discussed.
在自然生态系统中，微生物活动通常与表面的存在相关联，尤其是在低营养环境中。恒化器允许对这种低营养环境进行研究，并精确控制其他生长参数。通过使用该系统，已从两个不同河流来源的接种物中制备了富集培养物。当培养物受到碳限制时，与受到氮限制时相比，更多样化的群落附着在置于恒化器中的表面上。对从碳限制富集培养物中分离出的假单胞菌的进一步研究表明，与表面相关的生物体的生长速度约为其在周围自由培养基中生长速度的两倍。本文讨论了基于化学渗透理论来解释这一现象的假说。
