豌豆（Pisum sativum cv. Meteor）根中亚硝酸盐还原酶的纯化和性质。

Purification and properties of nitrite reductase from roots of pea (Pisum sativum cv. Meteor).

机构信息

Department of Agricultural Sciences, University of Bristol, Institute of Arable Crop Research, Long Ashton Research Station, Long Ashton, BS18 9AF, Bristol, UK.

出版信息

Planta. 1988 Sep;175(3):334-40. doi: 10.1007/BF00396338.

DOI:10.1007/BF00396338

PMID:24221870

Abstract

Nitrite reductase (EC 1.6.6.4) prepared from pea roots was found to be immunologically indistinguishable from pea leaf nitrite reductase. Comparisons of the pea root enzyme with nitrite reductase from leaf sources showed a close similarity in inhibition properties, light absorption spectrum, and electron paramagnetic resonance signals. The resemblances indicate that the root nitrite reductase is a sirohaem enzyme and that it functions in the same manner as the leaf enzyme in spite of the difference in reductant supply implicit in its location in a non-photosynthetic tissue.

摘要

从豌豆根部分离得到的亚硝酸盐还原酶（EC 1.6.6.4）在免疫学上与豌豆叶片亚硝酸盐还原酶无法区分。将豌豆根酶与叶片来源的亚硝酸盐还原酶进行比较，发现它们在抑制特性、光吸收光谱和电子顺磁共振信号方面非常相似。这些相似之处表明，根中的亚硝酸盐还原酶是一种亚铁血红素酶，尽管它位于非光合组织中，还原剂的供应存在差异，但它的功能与叶片中的酶相同。