Department of Biochemistry, Michigan State University, 48824, East Lansing, MI, USA.
Planta. 1988 Sep;175(3):389-95. doi: 10.1007/BF00396345.
The non-invasive technique of fluorescence redistribution after photobleaching was employed on soybean (Glycine max (L.) Merr.) root cells grown in suspension culture to examine macromolecular transport across plant cell walls. Using both fluorescently derivatized dextrans and proteins of graded size, a functional range of diameters for putative trans-wall channels was determined to be 6.6-8.6 nm. A mild treatment with pectinase apparently enlarged the channels, without adversely affecting cell viability, enabling significantly larger molecules to pass through the wall. Treatment of the cells with cellulysin or protease did not have this enlargement effect. It appears that the organization of pectic substances is a major control element in defining the sieving properties of the wall.
采用光漂白后荧光再分布的非侵入性技术研究了在悬浮培养中生长的大豆（Glycine max（L.）Merr.）根细胞中的大分子跨植物细胞壁运输。使用荧光衍生的葡聚糖和不同大小的蛋白质，确定了推测的跨壁通道的功能直径范围为 6.6-8.6nm。用果胶酶进行轻度处理显然会扩大通道，而不会对细胞活力产生不利影响，从而使更大的分子能够穿过细胞壁。用纤维素酶或蛋白酶处理细胞不会产生这种扩大效果。看来，果胶物质的组织是决定细胞壁筛孔特性的主要控制因素。
