John Innes Institute, Colney Lane, NR4 7UH, Norwich, UK.
Planta. 1977 Jan;133(3):267-73. doi: 10.1007/BF00380688.
The effect of including coumarin in the culture medium of isolated tobacco leaf protoplasts has been studied by scanning microscopy. Fibre formation is completely suppressed for 48 h in concentrations of 200 and 250 mg/l of coumarin. Continuous culture in these concentrations of coumarin does not result in cell division or colony formation, although an atypical wall is visible after 4 days. The effect of coumarin is reversible. Viability of protoplasts exposed for 48 h to 200 mg/l coumarin is unaffected in terms of plating efficiency and infectability with virus. These results are discussed in relation to cell wall synthesis and the possible use of coumarin in prolonging the protoplast state.
采用扫描电子显微镜研究了在离体烟草叶原生质体培养基中加入香豆素的效果。浓度为 200 和 250 毫克/升的香豆素可完全抑制纤维形成 48 小时。在这些浓度的香豆素中连续培养不会导致细胞分裂或集落形成，尽管在 4 天后可以看到非典型的细胞壁。香豆素的作用是可逆的。暴露于 200 毫克/升香豆素 48 小时的原生质体的存活率在平板效率和病毒感染性方面不受影响。这些结果与细胞壁合成有关，并讨论了香豆素在延长原生质体状态方面的可能用途。
