Brayton P R, Bode R B, Colwell R R, MacDonell M T, Hall H L, Grimes D J, West P A, Bryant T N
J Clin Microbiol. 1986 Jan;23(1):104-8. doi: 10.1128/jcm.23.1.104-108.1986.
A halophilic gram-negative rod was isolated from blood and cerebrospinal fluid collected from a 70-year-old male having no known contact with seafood or salt water. Positive biochemical tests included oxidase, sensitivity to 0/129, O-nitrophenyl-beta-D-galactopyranoside, lysine decarboxylase and fermentation of glucose, salicin, n-inositol, sucrose, L-mannose, L-arabinose, and arbutin. Negative tests included indole, ornithine decarboxylase, arginine dihydrolase fermentation of lactose, and production of gelatinase and urease. The DNA base composition was 45.0 mol% guanine plus cytosine. Numerical taxonomy indicated 70% similarity with known reference Vibrio sp. strains. The 5S rRNA sequence for this strain has been determined: 5'-U G C C U G G C G A C C A U A G C G U U U U G G A C C C A C C U G A U U C C A U G C C G A A C U C A G U A G U G A A A C G A A A C A G C G U C G A U G G U A G U G U G G G G U C U C C C C A U G U G A G A G U A G A A C A U C G C C A G G C A U-3'. Based on the phenetic, molecular genetic, and nucleic acid sequencing data, it is concluded that Vibrio cincinnatiensis represents a new species of the genus Vibrio sensu strictu (as defined by 5S rRNA sequencing results). On a basis of 5S rRNA comparative sequence analysis, the organism appears to share a recent common ancestor with V. gazogenes (98% homology) and close ancestry with V. mimicus, V. fluvialis, and V. metschnikovii.
从一名70岁男性的血液和脑脊液中分离出一株嗜盐革兰氏阴性杆菌，该男性无已知的海鲜或盐水接触史。阳性生化试验包括氧化酶试验、对0/129的敏感性试验、O-硝基苯基-β-D-吡喃半乳糖苷试验、赖氨酸脱羧酶试验以及葡萄糖、水杨苷、肌醇、蔗糖、L-甘露糖、L-阿拉伯糖和熊果苷发酵试验。阴性试验包括吲哚试验、鸟氨酸脱羧酶试验、精氨酸双水解酶试验、乳糖发酵试验以及明胶酶和脲酶产生试验。DNA碱基组成为45.0摩尔%的鸟嘌呤加胞嘧啶。数值分类表明与已知参考弧菌属菌株有70%的相似性。已确定该菌株的5S rRNA序列：5'-UGCCUGGCGA CC AUA GCGUUUU GGACC CACCUGA UUCCAUGCCGA ACUCAGUAGUGAA A C GAA ACA GCGUCG AUGGUAGUGUGGGGU CUC CCCAUGUGAGAGUAGA ACAUCGCCAGGCAU-3'。基于表型、分子遗传学和核酸测序数据，得出结论：辛辛那提弧菌代表弧菌属狭义定义（由5S rRNA测序结果定义）中的一个新物种。基于5S rRNA比较序列分析，该生物体似乎与产气弧菌有最近的共同祖先（98%同源性），并与模仿弧菌、河流弧菌和梅契尼科夫弧菌有密切的亲缘关系。
