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展示细菌鞭毛的实用方法。

Practical procedure for demonstrating bacterial flagella.

作者信息

Kodaka H, Armfield A Y, Lombard G L, Dowell V R

出版信息

J Clin Microbiol. 1982 Nov;16(5):948-52. doi: 10.1128/jcm.16.5.948-952.1982.

DOI:10.1128/jcm.16.5.948-952.1982
PMID:6185531
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC272507/
Abstract

We developed a simple, rapid method for demonstrating bacterial flagella with Ryu staining solution that gave satisfactory results for numerous motile and nonmotile bacteria. Two major advantages of this method are that the staining solution, ready for use, is stable at ambient temperature indefinitely and that microscopic examination of bacteria in the stained drop preparations can be performed rapidly.

摘要

我们开发了一种简单、快速的方法,用柳氏染色液显示细菌鞭毛,该方法对众多运动性和非运动性细菌均能给出满意结果。该方法的两个主要优点是,即用型染色液在室温下可无限期稳定保存,并且可以快速对染色悬滴标本中的细菌进行显微镜检查。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4b/272507/d7c802c9047f/jcm00148-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4b/272507/e51c09fee7b2/jcm00148-0199-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4b/272507/80bf3b1a639e/jcm00148-0200-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4b/272507/d7c802c9047f/jcm00148-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4b/272507/e51c09fee7b2/jcm00148-0199-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4b/272507/80bf3b1a639e/jcm00148-0200-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4b/272507/d7c802c9047f/jcm00148-0201-a.jpg

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Practical procedure for demonstrating bacterial flagella.展示细菌鞭毛的实用方法。
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A METHOD OF STAINING BACTERIAL FLAGELLA AND CAPSULES TOGETHER WITH A STUDY OF THE ORIGIN OF FLAGELLA.一种同时对细菌鞭毛和荚膜进行染色的方法以及对鞭毛起源的研究
J Bacteriol. 1930 Sep;20(3):203-11. doi: 10.1128/jb.20.3.203-211.1930.
2
Staining, shape and arrangement of bacterial flagella.细菌鞭毛的染色、形态及排列
J Bacteriol. 1951 Oct;62(4):377-89. doi: 10.1128/jb.62.4.377-389.1951.
3
Rapid flagella stain.快速鞭毛染色。
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SCC 3193 Mutants with Altered Synthesis of Cell Surface Polysaccharides Are Resistant to N4-Like Lytic Bacteriophage ϕA38 (vB_Ppp_A38) but Express Decreased Virulence in Potato ( L.) Plants.SCC 3193 突变体改变了细胞表面多糖的合成,对 N4 样裂解噬菌体 ϕA38(vB_Ppp_A38)具有抗性,但在马铃薯(L.)植物中表达的毒力降低。
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