MacDonell M T, Colwell R R
Appl Environ Microbiol. 1984 Jul;48(1):119-21. doi: 10.1128/aem.48.1.119-121.1984.
Enzymatic determination of the nucleotide sequence of 5S rRNA prepared from a Vibrio cholerae non-O1 serovar isolated from a canal in Louisiana in 1978 revealed it to be identical to that of V. cholerae O1 isolated in 1905 from a pilgrim at the E1 Tor quarantine station. The two strains are associated with sharply contrasting habitats. Ranges of variation of 5 to 15% in procaryotic 5S rRNA sequences between species are typical; variation within species may be 2% or greater. Because the 5S rRNA sequences were identical, it is concluded that they have a common phylogenic origin.
对1978年从路易斯安那州一条运河分离出的霍乱弧菌非O1血清型所制备的5S rRNA进行酶促核苷酸序列测定,结果显示其与1905年从托尔隔离站一名朝圣者身上分离出的霍乱弧菌O1血清型的5S rRNA序列相同。这两种菌株所处的栖息地截然不同。物种间原核生物5S rRNA序列的变异范围在5%至15%之间是典型的;物种内的变异可能为2%或更大。由于5S rRNA序列相同,因此得出结论,它们有共同的系统发育起源。