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Genetic diversity and virulence potential of environmental Vibrio cholerae population in a cholera-endemic area.霍乱流行地区环境中霍乱弧菌种群的遗传多样性和毒力潜力
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本文引用的文献

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A new method for the isolation of ribonucleic acids from mammalian tissues.一种从哺乳动物组织中分离核糖核酸的新方法。
Biochem J. 1956 Nov;64(3):405-8. doi: 10.1042/bj0640405.
2
Cholera--a possible endemic focus in the United States.霍乱——美国境内一个可能的地方性疫源地。
N Engl J Med. 1980 Feb 7;302(6):305-9. doi: 10.1056/NEJM198002073020601.
3
Occurrence of Vibrio cholerae serotype O1 in Maryland and Louisiana estuaries.马里兰州和路易斯安那州河口霍乱弧菌O1血清型的出现情况。
Appl Environ Microbiol. 1981 Feb;41(2):555-8. doi: 10.1128/aem.41.2.555-558.1981.
4
Sequences of the 5S rRNAs of the thermo-acidophilic archaebacterium Sulfolobus solfataricus (Caldariella acidophila) and the thermophilic eubacteria Bacillus acidocaldarius and Thermus aquaticus.嗜热嗜酸古细菌嗜热栖热菌（嗜酸栖热菌）、嗜热真细菌嗜酸芽孢杆菌和水生栖热菌的5S核糖体RNA序列。
Nucleic Acids Res. 1983 Jul 25;11(14):4667-76. doi: 10.1093/nar/11.14.4667.
5
Consensus structure and evolution of 5S rRNA.5S核糖体RNA的共有结构与进化
Nucleic Acids Res. 1983 Feb 11;11(3):893-900. doi: 10.1093/nar/11.3.893.
6
Phylogenetic tree derived from bacterial, cytosol and organelle 5S rRNA sequences.源自细菌、胞质溶胶和细胞器5S核糖体RNA序列的系统发育树。
Nucleic Acids Res. 1981 Mar 25;9(6):1451-61. doi: 10.1093/nar/9.6.1451.
7
The phylogeny of prokaryotes.原核生物的系统发育。
Science. 1980 Jul 25;209(4455):457-63. doi: 10.1126/science.6771870.
8
Open and closed 5 S ribosomal RNA, the only two universal structures encoded in the nucleotide sequences.开放型和封闭型5S核糖体RNA，是核苷酸序列中仅有的两种通用结构。
J Mol Biol. 1983 Sep 5;169(1):1-13. doi: 10.1016/s0022-2836(83)80172-7.
9
Use of solubilizable acrylamide disulfide gels for isolation of DNA fragments suitable for sequence analysis.可溶解的丙烯酰胺二硫化物凝胶用于分离适合序列分析的DNA片段。
Anal Biochem. 1981 Sep 1;116(1):146-51. doi: 10.1016/0003-2697(81)90337-7.
10
Purification and some properties of a non-o1 Vibrio cholerae enterotoxin that is identical to cholera enterotoxin.一种与霍乱肠毒素相同的非O1群霍乱弧菌肠毒素的纯化及其某些特性
Infect Immun. 1983 Mar;39(3):1128-35. doi: 10.1128/iai.39.3.1128-1135.1983.

代表时间、地理和生态多样性的霍乱弧菌菌株具有相同的5S rRNA核苷酸序列。

Identical 5S rRNA nucleotide sequence of Vibrio cholerae strains representing temporal, geographical, and ecological diversity.

作者信息

MacDonell M T, Colwell R R

出版信息

Appl Environ Microbiol. 1984 Jul;48(1):119-21. doi: 10.1128/aem.48.1.119-121.1984.

DOI:10.1128/aem.48.1.119-121.1984
PMID:6206795
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC240332/
Abstract

Enzymatic determination of the nucleotide sequence of 5S rRNA prepared from a Vibrio cholerae non-O1 serovar isolated from a canal in Louisiana in 1978 revealed it to be identical to that of V. cholerae O1 isolated in 1905 from a pilgrim at the E1 Tor quarantine station. The two strains are associated with sharply contrasting habitats. Ranges of variation of 5 to 15% in procaryotic 5S rRNA sequences between species are typical; variation within species may be 2% or greater. Because the 5S rRNA sequences were identical, it is concluded that they have a common phylogenic origin.

摘要

对1978年从路易斯安那州一条运河分离出的霍乱弧菌非O1血清型所制备的5S rRNA进行酶促核苷酸序列测定，结果显示其与1905年从托尔隔离站一名朝圣者身上分离出的霍乱弧菌O1血清型的5S rRNA序列相同。这两种菌株所处的栖息地截然不同。物种间原核生物5S rRNA序列的变异范围在5%至15%之间是典型的；物种内的变异可能为2%或更大。由于5S rRNA序列相同，因此得出结论，它们有共同的系统发育起源。