Jin X X, Zhao S L, Wang R X
Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, China.
Genet Mol Res. 2013 Oct 15;12(4):4575-8. doi: 10.4238/2013.October.15.6.
A pair of new universal 12S mitochondrial rRNA gene primers was designed through multiple alignment analysis of the mitochondrial tRNA(Phe) and the 5' region of 16S mitochondrial rRNA genes of different kinds of fishes. The primers were successfully used to amplify an expected product fragment of about 1.2 kb from various marine fish species, and the amplified DNA fragment was recognized to contain the complete 12S mitochondrial rRNA and tRNA(Val) genes, as well as a partial 16S mitochondrial rRNA gene of about 146 bp in length. The primers would facilitate the study of the species discrimination, population and evolution in marine fish species.
通过对不同鱼类线粒体tRNA(Phe)和16S线粒体rRNA基因5'区域的多重比对分析，设计了一对新的通用12S线粒体rRNA基因引物。这些引物成功地从各种海洋鱼类物种中扩增出约1.2 kb的预期产物片段，并且扩增的DNA片段被认为包含完整的12S线粒体rRNA和tRNA(Val)基因，以及长度约为146 bp的部分16S线粒体rRNA基因。这些引物将有助于海洋鱼类物种的种类鉴别、种群和进化研究。
