Herraez-Hernandez Elsa, Preda Ovidiu, Alonso Sonia, Pardo Rosario Serrano, Olmo Asuncion
R&D Department, Master Diagnóstica, Avenida del Conocimiento 100, Parque Tecnológico de Ciencias de la Salud, 18016, Granada, Spain.
Open Virol J. 2013 Oct 18;7:91-5. doi: 10.2174/1874357920130927004. eCollection 2013.
The novel HPV Direct Flow CHIP commercial system for Human Papillomavirus (HPV) genotyping is based on rapid PCR and automatic reverse dot blot hybridization to genotype-specific probes, allowing the detection of 36 HPV genotypes. This study examined the performance of HPV Direct Flow CHIP in formalin-fixed paraffin-embedded (FFPE) samples (n= 99). Each sample was analyzed both by Direct PCR, using crude cell extracts without DNA purification, and by conventional PCR, using purified DNA. Pair-wise analysis of the results demonstrated strong concordance between the results obtained with the two protocols, although a slightly higher rate of multiple infections was detected by conventional PCR. In summary, HPV Direct Flow CHIP achieves effective HPV detection from FFPE samples with both Direct PCR and Conventional PCR protocols.
新型人乳头瘤病毒（HPV）直接流式芯片商业系统用于人乳头瘤病毒基因分型，该系统基于快速聚合酶链反应（PCR）和与基因型特异性探针的自动反向点杂交，可检测36种HPV基因型。本研究检测了HPV直接流式芯片在福尔马林固定石蜡包埋（FFPE）样本（n = 99）中的性能。每个样本均通过直接PCR（使用未经DNA纯化的粗细胞提取物）和常规PCR（使用纯化的DNA）进行分析。结果的成对分析表明，两种方法所得结果之间具有高度一致性，尽管常规PCR检测到的多重感染率略高。总之，HPV直接流式芯片通过直接PCR和常规PCR方法均可从FFPE样本中有效检测HPV。
