Bani-Sacchi T, Barattini M, Bianchi S, Blandina P, Brunelleschi S, Fantozzi R, Mannaioni P F, Masini E
J Physiol. 1986 Feb;371:29-43. doi: 10.1113/jphysiol.1986.sp015960.
Two preparations, a segment of rat ileum and the vagally innerved guinea-pig auricles, have been used in an analysis of the responses to vagal or to electrical field stimulation. The responses to parasympathetic stimulation were depressed by atropine and by tetrodotoxin, and potentiated by eserine. Supramaximal stimulation (10-20 Hz) resulted in increased release of acetylcholine and histamine, both in rat ileum and guinea-pig auricles. The release of histamine after parasympathetic stimulation did not exhibit tachyphylaxis, and it was not reproduced by non-parasympathetic stimuli. In both preparations, atropine produced a significant, dose-related reduction of histamine measured in the bath fluid after stimulation, while eserine increased histamine output. A significant diminution of mast cell granules metachromasia was observed in guinea-pig auricles and in rat intestine after parasympathetic stimulation. The possibility is discussed that acetylcholine released by parasympathetic stimulation would in turn evoke the secretion of histamine from tissue mast cells.
为了分析对迷走神经或电场刺激的反应，采用了两种标本，即一段大鼠回肠和有迷走神经支配的豚鼠耳廓。副交感神经刺激的反应可被阿托品和河豚毒素抑制，并被毒扁豆碱增强。在大鼠回肠和豚鼠耳廓中，超最大刺激（10 - 20赫兹）均导致乙酰胆碱和组胺释放增加。副交感神经刺激后组胺的释放未表现出快速耐受性，且非副交感神经刺激不能使其重现。在两种标本中，阿托品使刺激后浴液中测得的组胺显著减少，且呈剂量相关，而毒扁豆碱增加了组胺的释放量。在豚鼠耳廓和大鼠肠道中，副交感神经刺激后观察到肥大细胞颗粒异染性显著降低。文中讨论了副交感神经刺激释放的乙酰胆碱反过来会诱发组织肥大细胞分泌组胺的可能性。
