Sawada S, Shida M, Suenaga R, Mizuma H, Karasaki M, Hashimoto M, Kawano K, Amaki I
J Clin Lab Immunol. 1986 Jan;19(1):31-5.
Sera from 7 patients with multiple myeloma having antistreptolysin O (ASO) activity in high titers were detected by a streptolysin O (SLO) inhibition assay. However, activity was in low titer when assayed by a passive agglutination assay. The discrepancy between these 2 assays raised some doubts as to whether these monoclonal proteins (M.protein) bond to SLO in the same manner as elicited antibodies. Immunochemical analysis and idiotope analysis using monoclonal antibody to one of these M.proteins strongly suggest that M.protein with ASO activity bind to SLO in a manner similar to elicited antibody. The discrepancy between the 2 assays might be due to differences in the antigenic structure of different forms of the SLO molecule.
采用抗链球菌溶血素O（SLO）抑制试验检测了7例多发性骨髓瘤患者血清，这些血清具有高滴度的抗链球菌溶血素O（ASO）活性。然而，采用被动凝集试验检测时活性滴度较低。这两种检测方法之间的差异引发了一些疑问，即这些单克隆蛋白（M蛋白）是否以与诱导抗体相同的方式与SLO结合。使用针对其中一种M蛋白的单克隆抗体进行免疫化学分析和独特型分析强烈表明，具有ASO活性的M蛋白以与诱导抗体相似的方式与SLO结合。这两种检测方法之间的差异可能是由于SLO分子不同形式的抗原结构差异所致。
