• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

CARMA1 CARD的晶体结构揭示了CARD结构域新型二硫键介导的二聚化。

Novel disulfide bond-mediated dimerization of the CARD domain was revealed by the crystal structure of CARMA1 CARD.

作者信息

Jang Tae-ho, Park Jin Hee, Park Hyun Ho

机构信息

School of Biotechnology and Graduate School of Biochemistry at Yeungnam University, Gyeongsan, South Korea.

出版信息

PLoS One. 2013 Nov 5;8(11):e79778. doi: 10.1371/journal.pone.0079778. eCollection 2013.

DOI:10.1371/journal.pone.0079778
PMID:24224005
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3818214/
Abstract

CARMA1, BCL10 and MALT1 form a large molecular complex known as the CARMA1 signalosome during lymphocyte activation. Lymphocyte activation via the CARMA1 signalosome is critical to immune response and linked to many immune diseases. Despite the important role of the CARMA1 signalosome during lymphocyte activation and proliferation, limited structural information is available. Here, we report the dimeric structure of CARMA1 CARD at a resolution of 3.2 Å. Interestingly, although CARMA1 CARD has a canonical six helical-bundles structural fold similar to other CARDs, CARMA1 CARD shows the first homo-dimeric structure of CARD formed by a disulfide bond and reveals a possible biologically important homo-dimerization mechanism.

摘要

在淋巴细胞激活过程中，CARMA1、BCL10和MALT1形成一种称为CARMA1信号小体的大分子复合物。通过CARMA1信号小体进行的淋巴细胞激活对免疫反应至关重要，并与许多免疫疾病相关。尽管CARMA1信号小体在淋巴细胞激活和增殖过程中发挥着重要作用，但可用的结构信息有限。在此，我们报告了分辨率为3.2 Å的CARMA1 CARD的二聚体结构。有趣的是，尽管CARMA1 CARD具有与其他CARD类似的典型六螺旋束结构折叠，但CARMA1 CARD显示出由二硫键形成的CARD的首个同型二聚体结构，并揭示了一种可能具有生物学重要性的同型二聚化机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de9c/3818214/1fa017aabece/pone.0079778.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de9c/3818214/a552d181059b/pone.0079778.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de9c/3818214/9f13ab3d0373/pone.0079778.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de9c/3818214/fec21a9207ba/pone.0079778.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de9c/3818214/473fe02ca6b7/pone.0079778.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de9c/3818214/1fa017aabece/pone.0079778.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de9c/3818214/a552d181059b/pone.0079778.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de9c/3818214/9f13ab3d0373/pone.0079778.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de9c/3818214/fec21a9207ba/pone.0079778.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de9c/3818214/473fe02ca6b7/pone.0079778.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de9c/3818214/1fa017aabece/pone.0079778.g005.jpg

相似文献

1
Novel disulfide bond-mediated dimerization of the CARD domain was revealed by the crystal structure of CARMA1 CARD.CARMA1 CARD的晶体结构揭示了CARD结构域新型二硫键介导的二聚化。
PLoS One. 2013 Nov 5;8(11):e79778. doi: 10.1371/journal.pone.0079778. eCollection 2013.
2
Self-oligomerization of the CARD domain prevents complex formation in the CARMA1 signalosome.CARD 结构域的自身寡聚化阻止了 CARMA1 信号体复合物的形成。
Int J Mol Med. 2013 May;31(5):1280-7. doi: 10.3892/ijmm.2013.1307. Epub 2013 Mar 19.
3
Structural insights into the assembly of CARMA1 and BCL10.CARMA1 和 BCL10 组装的结构见解。
PLoS One. 2012;7(8):e42775. doi: 10.1371/journal.pone.0042775. Epub 2012 Aug 3.
4
Structural architecture of the CARMA1/Bcl10/MALT1 signalosome: nucleation-induced filamentous assembly.CARMA1/Bcl10/MALT1 信号体的结构架构：引发丝状体组装的成核作用。
Mol Cell. 2013 Sep 26;51(6):766-79. doi: 10.1016/j.molcel.2013.08.032.
5
Assembly mechanism of the CARMA1-BCL10-MALT1-TRAF6 signalosome.CARMA1-BCL10-MALT1-TRAF6 信号小体的组装机制。
Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):1499-1504. doi: 10.1073/pnas.1721967115. Epub 2018 Jan 30.
6
Crystallization and preliminary X-ray crystallographic studies of the CARD domain of human CARMA1.人CARMA1的CARD结构域的结晶及初步X射线晶体学研究。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Apr 1;69(Pt 4):435-7. doi: 10.1107/S1744309113005642. Epub 2013 Mar 28.
7
Protein kinase C-δ negatively regulates T cell receptor-induced NF-κB activation by inhibiting the assembly of CARMA1 signalosome.蛋白激酶 C-δ 通过抑制 CARMA1 信号体的组装来负调控 T 细胞受体诱导的 NF-κB 激活。
J Biol Chem. 2012 Jun 8;287(24):20081-7. doi: 10.1074/jbc.M111.335463. Epub 2012 Apr 23.
8
MAGUK-controlled ubiquitination of CARMA1 modulates lymphocyte NF-kappaB activity.MAGUK 调控的 CARMA1 泛素化调节淋巴细胞 NF-κB 活性。
Mol Cell Biol. 2010 Feb;30(4):922-34. doi: 10.1128/MCB.01129-09. Epub 2009 Dec 14.
9
Role of the CARMA1/BCL10/MALT1 complex in lymphoid malignancies.CARMA1/BCL10/MALT1复合物在淋巴系统恶性肿瘤中的作用。
Curr Opin Hematol. 2016 Jul;23(4):402-9. doi: 10.1097/MOH.0000000000000257.
10
In vitro reconstitution of interactions in the CARD9 signalosome.在体外重建 CARD9 信号体中的相互作用。
Mol Med Rep. 2017 Oct;16(4):3910-3916. doi: 10.3892/mmr.2017.7116. Epub 2017 Jul 31.

引用本文的文献

1
Assembly of platforms for signal transduction in the new era: dimerization, helical filament assembly, and beyond.新时代信号转导平台的组装：二聚化、螺旋丝组装及其他。
Exp Mol Med. 2020 Mar;52(3):356-366. doi: 10.1038/s12276-020-0391-3. Epub 2020 Mar 5.
2
Caspase recruitment domains for protein interactions in cellular signaling (Review).细胞信号转导中蛋白质相互作用的半胱氨酸天冬氨酸蛋白酶募集结构域（综述）。
Int J Mol Med. 2019 Mar;43(3):1119-1127. doi: 10.3892/ijmm.2019.4060. Epub 2019 Jan 11.
3
Mechanisms of Regulated and Dysregulated CARD11 Signaling in Adaptive Immunity and Disease.

本文引用的文献

1
Crystallization and preliminary X-ray crystallographic studies of the CARD domain of human CARMA1.人CARMA1的CARD结构域的结晶及初步X射线晶体学研究。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Apr 1;69(Pt 4):435-7. doi: 10.1107/S1744309113005642. Epub 2013 Mar 28.
2
Structural insights into the assembly of CARMA1 and BCL10.CARMA1 和 BCL10 组装的结构见解。
PLoS One. 2012;7(8):e42775. doi: 10.1371/journal.pone.0042775. Epub 2012 Aug 3.
3
A comprehensive manually curated protein-protein interaction database for the Death Domain superfamily.
调节性和失调性 CARD11 信号在适应性免疫和疾病中的作用机制。
Front Immunol. 2018 Sep 19;9:2105. doi: 10.3389/fimmu.2018.02105. eCollection 2018.
4
Molecular architecture and regulation of BCL10-MALT1 filaments.BCL10-MALT1 丝状体的分子结构与调控。
Nat Commun. 2018 Oct 2;9(1):4041. doi: 10.1038/s41467-018-06573-8.
5
Crystal structure of human NLRP12 PYD domain and implication in homotypic interaction.人NLRP12吡咯结构域的晶体结构及其在同型相互作用中的意义。
PLoS One. 2018 Jan 2;13(1):e0190547. doi: 10.1371/journal.pone.0190547. eCollection 2018.
6
Crystal structure of caspase recruiting domain (CARD) of apoptosis repressor with CARD (ARC) and its implication in inhibition of apoptosis.含半胱天冬酶募集结构域的凋亡抑制因子（ARC）的半胱天冬酶募集结构域（CARD）的晶体结构及其在抑制细胞凋亡中的作用
Sci Rep. 2015 Jun 3;5:9847. doi: 10.1038/srep09847.
7
Crystallization and preliminary X-ray crystallographic analysis of the CARD domain of apoptosis repressor with CARD (ARC).含CARD结构域的凋亡抑制蛋白（ARC）的CARD结构域的结晶及初步X射线晶体学分析
Acta Crystallogr F Struct Biol Commun. 2015 Jan 1;71(Pt 1):82-5. doi: 10.1107/S2053230X14026211.
8
An updated view on the structure and function of PYRIN domains.关于吡啉结构域的结构与功能的最新观点。
Apoptosis. 2015 Feb;20(2):157-73. doi: 10.1007/s10495-014-1065-1.
一个综合性的、经过人工精心策展的死亡结构域超家族蛋白质-蛋白质相互作用数据库。
Nucleic Acids Res. 2012 Jan;40(Database issue):D331-6. doi: 10.1093/nar/gkr1149. Epub 2011 Dec 1.
4
Antagonists induce a conformational change in cIAP1 that promotes autoubiquitination.拮抗剂诱导 cIAP1 构象变化，促进自身泛素化。
Science. 2011 Oct 21;334(6054):376-80. doi: 10.1126/science.1207862.
5
Structural basis for the activation of innate immune pattern-recognition receptor RIG-I by viral RNA.病毒 RNA 激活先天免疫模式识别受体 RIG-I 的结构基础。
Cell. 2011 Oct 14;147(2):423-35. doi: 10.1016/j.cell.2011.09.039.
6
Crystal structure of NALP3 protein pyrin domain (PYD) and its implications in inflammasome assembly.NALP3 蛋白的晶体结构 pyrin 域 (PYD) 及其在炎症小体组装中的意义。
J Biol Chem. 2011 Nov 11;286(45):39528-36. doi: 10.1074/jbc.M111.278812. Epub 2011 Aug 31.
7
Generalized semi-refolding methods for purification of the functional death domain superfamily.通用半重折叠方法用于功能死亡结构域超家族的纯化。
J Biotechnol. 2011 Feb 20;151(4):335-42. doi: 10.1016/j.jbiotec.2011.01.003. Epub 2011 Jan 13.
8
Structural analyses of death domains and their interactions.死亡结构域及其相互作用的结构分析。
Apoptosis. 2011 Mar;16(3):209-20. doi: 10.1007/s10495-010-0571-z.
9
Crystal structure of the Caenorhabditis elegans apoptosome reveals an octameric assembly of CED-4.秀丽隐杆线虫凋亡体的晶体结构揭示了 CED-4 的八聚体组装。
Cell. 2010 Apr 30;141(3):446-57. doi: 10.1016/j.cell.2010.03.017.
10
CARMA1-mediated NF-kappaB and JNK activation in lymphocytes.CARMA1介导淋巴细胞中的核因子κB和JNK激活。
Immunol Rev. 2009 Mar;228(1):199-211. doi: 10.1111/j.1600-065X.2008.00749.x.