Kim Seong Hyun, Park Hyun Ho
Department of Biochemistry, Yeungnam University, Gyeongsan, Republic of Korea.
Acta Crystallogr F Struct Biol Commun. 2015 Jan 1;71(Pt 1):82-5. doi: 10.1107/S2053230X14026211.
Apoptosis repressor with caspase-recruiting domain (ARC) is an apoptosis repressor that inhibits both intrinsic and extrinsic apoptosis signalling. Human ARC contains an N-terminal caspase-recruiting domain (CARD domain) and a C-terminal proline- and glutamic acid-rich (P/E-rich) domain. The CARD domain in ARC is the domain that is directly involved in inhibition of the extrinsic pathway. In this study, the N-terminal CARD domain of ARC was overexpressed, purified and crystallized. X-ray diffraction data were collected to a resolution of 2.1 Å and the crystals were found to belong to space group P6(1) or P65, with unit-cell parameters a=98.28, b=98.28, c=51.86 Å, α=90, β=90, γ=120°.
含半胱天冬酶招募结构域的凋亡抑制因子（ARC）是一种凋亡抑制因子，可抑制内源性和外源性凋亡信号传导。人ARC包含一个N端半胱天冬酶招募结构域（CARD结构域）和一个C端富含脯氨酸和谷氨酸的结构域（富含P/E结构域）。ARC中的CARD结构域是直接参与抑制外源性途径的结构域。在本研究中，ARC的N端CARD结构域被过表达、纯化并结晶。收集到分辨率为2.1 Å的X射线衍射数据，发现晶体属于空间群P6(1)或P65，晶胞参数a=98.28，b=98.28，c=51.86 Å，α=90，β=90，γ=120°。
