Physiologie Cellulaire Végétale, DRF, Centre d'Etudes Nucléaires et Université Scientifique et Médicale, 85X F38041 GRENOBLE Cedex FRANCE.
Plant Physiol. 1985 Oct;79(2):458-67. doi: 10.1104/pp.79.2.458.
Isolated cauliflower (Brassica oleracea) bud plastids, purified by isopycnic centrifugation in density gradients of Percoll, were found to be highly intact, to be practically devoid of extraplastidial contaminations, and to retain all the enzymes involved in fatty acid, phosphatidic acid, and monogalactosyldiacylglycerol synthesis. Purified plastids possess all the enzymes needed to convert triose phosphate to starch and vice versa, and are capable of conversion of glycerate 3-phosphate to pyruvate for fatty acid synthesis. They are also capable of oxidation of hexose phosphate and conversion to triose phosphate via the oxidative pentosephosphate pathway. Cauliflower bud plastids prove to be, therefore, biochemically very flexible organelles.
经等密度离心 Percoll 密度梯度纯化的离体花椰菜（甘蓝型油菜）芽质体高度完整，几乎不含质体外杂质，并保留了参与脂肪酸、磷脂酸和单半乳糖二酰基甘油合成的所有酶。纯化的质体具有将三磷酸丙糖转化为淀粉和反之亦然所需的所有酶，并且能够将甘油酸 3-磷酸转化为丙酮酸以进行脂肪酸合成。它们还能够氧化己糖磷酸并通过氧化戊糖磷酸途径转化为三磷酸丙糖。因此，花椰菜芽质体被证明是生化上非常灵活的细胞器。
