Department of Biological Sciences, University of Durham, South Road, DH1 3LE, Durham, UK.
Plant Cell Rep. 1990 Jul;9(2):88-92. doi: 10.1007/BF00231556.
Co-transformation of tobacco (Nicotiana tabacum) leaf explants with Agrobacterium rhizogenes harbouring pRi1855 and the binary vector pBin19 was achieved at a frequency of 67%. The kanamycin resistant hairy roots were cultured via a callusing phase to regenerate plants which were partially fertile when outcrossed with wild-type pollen. Phenotypic and molecular analysis of the F1 progeny demonstrated the efficient segregation of the hairy root marker from the kanamycin resistance marker, enabling morphologically normal plants to be recovered which retained the binary vector marker gene. This co-transformation strategy provides a means of introducing non-selectable genes into plants in cases where antibiotic resistance markers are undesirable.
农杆菌 rhizogenes harbouring pRi1855 和二元载体 pBin19 共转化烟草（Nicotiana tabacum）叶片外植体的频率为 67%。通过愈伤组织培养阶段，对卡那霉素抗性毛状根进行培养，以再生与野生型花粉杂交时部分可育的植物。F1 后代的表型和分子分析表明，毛状根标记与卡那霉素抗性标记的有效分离，使能够回收具有二元载体标记基因的形态正常的植物。这种共转化策略为在抗生素抗性标记物不可取的情况下将非选择性基因引入植物提供了一种方法。
