Dipartimento di Genetica e Biologia Molecolare, Facoltà di Scienze, Università di Roma, 00100, Roma, Italy.
Plant Mol Biol. 1982 Dec;1(4):291-300. doi: 10.1007/BF00027560.
The region of the virulence plasmid of the agropine type Agrobacterium rhizogenes 1855 (pRi-1855) which is transferred to plant cells upon infection (T-DNA) has been identified by means of Southern blot hybridizations with the T-DNA of the mannopine type A. rhizogenes 8196. The presence in the plant genome of the pRi-1855 sequences thus identified is demonstrated in carrot roots derived from infection with strain 1855. The T-DNA of pRi-1855 has been mapped by means of cloned Eco RI partial digests. Although strongly homologous with each other, the cores of the T-regions of the mannopine and agropine Ri plasmids are not colinear since the latter contains a central segment of DNA which is absent from the T-region of pRi-8196. Unexpected homologies between the T-region of pRi-1855 and normal carrot DNA have been observed and are discussed here.
根癌农杆菌 1855 型（pRi-1855）的毒性质粒区域，在侵染植物细胞时被转移（T-DNA），通过与 mannopine 型根癌农杆菌 8196 的 T-DNA 的 Southern 印迹杂交得到了鉴定。在胡萝卜根中，通过感染菌株 1855 可以证明植物基因组中存在这些被鉴定的 pRi-1855 序列。通过克隆的 Eco RI 部分消化，pRi-1855 的 T-DNA 已经被作图。尽管 mannopine 和 agropine Ri 质粒的 T 区核心彼此之间具有很强的同源性，但后者包含一个中央 DNA 片段，该片段不存在于 pRi-8196 的 T 区中。因此，农杆菌 1855 型 Ri 质粒 T 区与正常胡萝卜 DNA 之间存在意想不到的同源性，本文对此进行了讨论。
