Department of Forest Genetics, Swedish University of Agricultural Sciences, Box 7027, S-750 07, Uppsala, Sweden.
Theor Appl Genet. 1990 May;79(5):654-6. doi: 10.1007/BF00226879.
The purpose of this study was to investigate the genetic variation of susceptibility to Agrobacterium within a Picea abies population. Tumor formation was studied in 16 open-pollinated families belonging to a central Swedish population of Picea abies. Strain A281 of Agrobacterium tumefaciens was used to infect 3-monthold seedlings in a five-block greenhouse experiment. The analysis of variance showed strong significance for the between-family variation of tumor-formation percentages, varying from 28% to 73%. The most susceptible material will be suitable for experiments on the production of transgenic plants in vitro using disarmed Agrobacterium strains.
本研究旨在调查白皮松群体对农杆菌易感性的遗传变异。在瑞典中部的白皮松种群中，我们对 16 个自由授粉家系进行了肿瘤形成研究。采用根癌农杆菌 A281 菌株在五个温室区块试验中感染 3 个月大的幼苗。方差分析显示，家系间肿瘤形成百分率的变异具有显著意义，从 28%到 73%不等。最易感的材料将适合使用无武装农杆菌菌株在体外进行转基因植物生产实验。
