Hood E E, Clapham D H, Ekberg I, Johannson T
Dept. of Forest Genetics, Swedish University of Agricultural Sciences, Uppsala.
Plant Mol Biol. 1990 Feb;14(2):111-7. doi: 10.1007/BF00018552.
The hypervirulent Agrobacterium tumefaciens strain A281 formed frequent tumors (31%) on Picea abies (Norway spruce), an economically important tree species in Swedish forests. Three-month-old seedlings were inoculated and tumors were established that grew hormone-independently in culture. Tumors contained agropine and mannopine/mannopinic acid as determined by acid pH paper electrophoresis. In addition, DNA hybridization studies showed that the DNA from these tumor lines contained sequences homologous to Ti plasmid T-DNA, whereas wild-type spruce seedling DNA did not. These results suggest that Agrobacterium vectors can be used for gene transfer into this important forest species.
超毒力根癌农杆菌菌株A281在瑞典森林中具有重要经济价值的树种欧洲云杉上频繁诱发肿瘤（发生率为31%）。对接种3个月大的幼苗后形成了肿瘤，这些肿瘤在培养过程中能够不依赖激素生长。通过酸性pH试纸电泳测定，肿瘤中含有章鱼碱和甘露碱/甘露碱酸。此外，DNA杂交研究表明，这些肿瘤系的DNA含有与Ti质粒T-DNA同源的序列，而野生型云杉幼苗的DNA则没有。这些结果表明，农杆菌载体可用于将基因导入这种重要的森林树种。
