Biological Laboratory, College of Education, Fukui University, 910, Fukui, Japan.
Plant Cell Rep. 1990 Jul;9(3):113-6. doi: 10.1007/BF00232083.
Protoplasts isolated enzymatically from juvenile leaflets of sporophytes in fern Lyqodium japonicum were inoculated in a MS medium containing NAA 2.7 μm, BAP 2.2 μm, 0.6 M mannitol, and 0.05 M sucrose. Cell division took place within 8 days of culture initiation, and after 30 days cell-clusters with 10-15 cells were observed. When the cell-clusters were transferred into fresh hormone-free medium containing no mannitol, they developed rhizoids and protonema-like regenerants. About 2 months later, cordate prothallia developed bearing both antheridia and archegonia.
从蕨类植物中华瓶尔小草的幼孢子体叶片中通过酶解分离得到的原生质体，接种在含有 NAA 2.7 μm、BAP 2.2 μm、0.6 M 甘露醇和 0.05 M 蔗糖的 MS 培养基中。培养起始后 8 天内发生细胞分裂，30 天后观察到有 10-15 个细胞的细胞团。当细胞团转移到不含甘露醇的新鲜无激素培养基中时，它们发育出假根和类似于原丝体的再生体。大约 2 个月后，心形原叶体发育成熟，具有精子器和颈卵器。
