Department of Biosciences, University of Jammu, 180004, Jammu, India.
Plant Cell Rep. 1990 Jul;9(3):137-8. doi: 10.1007/BF00232089.
Callus was obtained from mericarps of Bunium persicum Boiss. on MS medium supplemented with 2.0 mg/1 2,4-D and 4.0 mg/1 Kn. Small white clumps of compactly packed cells developed on the callus on a medium containing 1.0 mg/1 2,4-D and 0 mg/1 Kn. These cell clumps differentiated numerous globular embryos on the same medium. Embryo maturation and germination was achieved on the basal as well as on 1 mg/1 Kn supplemented medium. All regenerated plants examined were normal diploids with 2n=14.
愈伤组织从小茴香的瘦果中提取，在 MS 培养基上添加 2.0 mg/1 2,4-D 和 4.0 mg/1 Kn。在含有 1.0 mg/1 2,4-D 和 0 mg/1 Kn 的培养基上，愈伤组织上形成了小的白色块状紧密堆积的细胞。这些细胞团在相同的培养基上分化出许多球形胚胎。在基础培养基和添加 1 mg/1 Kn 的培养基上都可以实现胚胎成熟和萌发。所有检测到的再生植株均为正常二倍体，2n=14。
